Human epithelial cell processing of carbon and gold nanoparticles

Barbara J. Panessa-Warren, John B. Warren, Mathew M Maye, Daniel Van Der Lelie, Oleg Gang, Stanislaus S. Wong, Berhane Ghebrehiwet, George T. Tortora, James A. Misewich

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This paper describes some early cellular and intracellular interactions of human polarised lung and colon epithelial cells (representative of two portals of entry, inhalation and ingestion), following exposure to specific carbon and gold engineered nanoparticles in vitro. Cells were incubated with functionalised and non-functionalised carbon nanotube-derived nanoloops (-28-60 nm diameter), or gold nanoparticles (2 nm and 10 nm Au-core) which were either non-functionalised, or functionalised with biological proteins or ssDNA and analysed using viability staining, transmission electron microscopy (TEM) and field emission scanning (FESEM) electron microscopy. Even with such diverse nanoparticles and functionalisations, we found that the surface properties and size of the nanoparticles determined their cellular binding, incorporation and/or cytotoxicity. However the cells responded to the different types of nanoparticles using various intracellular routes which differed with the cell type, but all of the nanoparticles ultimately were consolidated into aggregates and transported to the basal cell surface. Nanoparticles that were completely covered with biological macromolecules (i.e., recombinant gClq-R protein, non-immune IgGk, monoclonal antibody to gClq-R, or ssDNA) did not cause ultrastructural damage or changes in the cell monolayers. Monoclonal antibody (mAb)-functionalised carbon nanoloops and ssDNA 100% covered Au-nanodots were incorporated and transported within the colon cells using different cellular pathways than those used by the lung cells. Citrate-capped Au-nanoparticles (2 nm and 10 nm) and 20% DNA covered Au-nanoparticles passed into the colon and lung cells through small holes in the apical cell membrane, which could possibly be produced by lipid peroxidation. Serious forms of cell damage were observed with citrate capped 2 nm and 10 nm Au-nanoparticles (i.e., nuclear localisation (2nm-Au); intracellular membrane damage (10 nm-Au)). Vital staining used to identify cellular necrosis following nanoparticle exposure, was sometimes misleading showing cell necrosis statistics similar to normal controls, when TEM analysis revealed intracellular and organellar damage in identically treated cells.

Original languageEnglish (US)
Pages (from-to)55-91
Number of pages37
JournalInternational Journal of Nanotechnology
Volume5
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

Fingerprint

Gold
Carbon
gold
Nanoparticles
nanoparticles
carbon
Processing
cells
lungs
damage
Monoclonal antibodies
necrosis
Cells
staining
citrates
antibodies
Citric Acid
Epithelial Cells
Monoclonal Antibodies
Transmission electron microscopy

Keywords

  • Antibody-functionalised
  • Carbon nanoparticles
  • Cellular processing
  • Cytotoxicity
  • DNA-functionalised
  • Gold nanoparticles

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Materials Science (miscellaneous)

Cite this

Panessa-Warren, B. J., Warren, J. B., Maye, M. M., Van Der Lelie, D., Gang, O., Wong, S. S., ... Misewich, J. A. (2008). Human epithelial cell processing of carbon and gold nanoparticles. International Journal of Nanotechnology, 5(1), 55-91. https://doi.org/10.1504/IJNT.2008.016549

Human epithelial cell processing of carbon and gold nanoparticles. / Panessa-Warren, Barbara J.; Warren, John B.; Maye, Mathew M; Van Der Lelie, Daniel; Gang, Oleg; Wong, Stanislaus S.; Ghebrehiwet, Berhane; Tortora, George T.; Misewich, James A.

In: International Journal of Nanotechnology, Vol. 5, No. 1, 01.2008, p. 55-91.

Research output: Contribution to journalArticle

Panessa-Warren, BJ, Warren, JB, Maye, MM, Van Der Lelie, D, Gang, O, Wong, SS, Ghebrehiwet, B, Tortora, GT & Misewich, JA 2008, 'Human epithelial cell processing of carbon and gold nanoparticles', International Journal of Nanotechnology, vol. 5, no. 1, pp. 55-91. https://doi.org/10.1504/IJNT.2008.016549
Panessa-Warren, Barbara J. ; Warren, John B. ; Maye, Mathew M ; Van Der Lelie, Daniel ; Gang, Oleg ; Wong, Stanislaus S. ; Ghebrehiwet, Berhane ; Tortora, George T. ; Misewich, James A. / Human epithelial cell processing of carbon and gold nanoparticles. In: International Journal of Nanotechnology. 2008 ; Vol. 5, No. 1. pp. 55-91.
@article{ea44a999db314871ab5ea996ff6e06bd,
title = "Human epithelial cell processing of carbon and gold nanoparticles",
abstract = "This paper describes some early cellular and intracellular interactions of human polarised lung and colon epithelial cells (representative of two portals of entry, inhalation and ingestion), following exposure to specific carbon and gold engineered nanoparticles in vitro. Cells were incubated with functionalised and non-functionalised carbon nanotube-derived nanoloops (-28-60 nm diameter), or gold nanoparticles (2 nm and 10 nm Au-core) which were either non-functionalised, or functionalised with biological proteins or ssDNA and analysed using viability staining, transmission electron microscopy (TEM) and field emission scanning (FESEM) electron microscopy. Even with such diverse nanoparticles and functionalisations, we found that the surface properties and size of the nanoparticles determined their cellular binding, incorporation and/or cytotoxicity. However the cells responded to the different types of nanoparticles using various intracellular routes which differed with the cell type, but all of the nanoparticles ultimately were consolidated into aggregates and transported to the basal cell surface. Nanoparticles that were completely covered with biological macromolecules (i.e., recombinant gClq-R protein, non-immune IgGk, monoclonal antibody to gClq-R, or ssDNA) did not cause ultrastructural damage or changes in the cell monolayers. Monoclonal antibody (mAb)-functionalised carbon nanoloops and ssDNA 100{\%} covered Au-nanodots were incorporated and transported within the colon cells using different cellular pathways than those used by the lung cells. Citrate-capped Au-nanoparticles (2 nm and 10 nm) and 20{\%} DNA covered Au-nanoparticles passed into the colon and lung cells through small holes in the apical cell membrane, which could possibly be produced by lipid peroxidation. Serious forms of cell damage were observed with citrate capped 2 nm and 10 nm Au-nanoparticles (i.e., nuclear localisation (2nm-Au); intracellular membrane damage (10 nm-Au)). Vital staining used to identify cellular necrosis following nanoparticle exposure, was sometimes misleading showing cell necrosis statistics similar to normal controls, when TEM analysis revealed intracellular and organellar damage in identically treated cells.",
keywords = "Antibody-functionalised, Carbon nanoparticles, Cellular processing, Cytotoxicity, DNA-functionalised, Gold nanoparticles",
author = "Panessa-Warren, {Barbara J.} and Warren, {John B.} and Maye, {Mathew M} and {Van Der Lelie}, Daniel and Oleg Gang and Wong, {Stanislaus S.} and Berhane Ghebrehiwet and Tortora, {George T.} and Misewich, {James A.}",
year = "2008",
month = "1",
doi = "10.1504/IJNT.2008.016549",
language = "English (US)",
volume = "5",
pages = "55--91",
journal = "International Journal of Nanotechnology",
issn = "1475-7435",
publisher = "Inderscience Enterprises Ltd",
number = "1",

}

TY - JOUR

T1 - Human epithelial cell processing of carbon and gold nanoparticles

AU - Panessa-Warren, Barbara J.

AU - Warren, John B.

AU - Maye, Mathew M

AU - Van Der Lelie, Daniel

AU - Gang, Oleg

AU - Wong, Stanislaus S.

AU - Ghebrehiwet, Berhane

AU - Tortora, George T.

AU - Misewich, James A.

PY - 2008/1

Y1 - 2008/1

N2 - This paper describes some early cellular and intracellular interactions of human polarised lung and colon epithelial cells (representative of two portals of entry, inhalation and ingestion), following exposure to specific carbon and gold engineered nanoparticles in vitro. Cells were incubated with functionalised and non-functionalised carbon nanotube-derived nanoloops (-28-60 nm diameter), or gold nanoparticles (2 nm and 10 nm Au-core) which were either non-functionalised, or functionalised with biological proteins or ssDNA and analysed using viability staining, transmission electron microscopy (TEM) and field emission scanning (FESEM) electron microscopy. Even with such diverse nanoparticles and functionalisations, we found that the surface properties and size of the nanoparticles determined their cellular binding, incorporation and/or cytotoxicity. However the cells responded to the different types of nanoparticles using various intracellular routes which differed with the cell type, but all of the nanoparticles ultimately were consolidated into aggregates and transported to the basal cell surface. Nanoparticles that were completely covered with biological macromolecules (i.e., recombinant gClq-R protein, non-immune IgGk, monoclonal antibody to gClq-R, or ssDNA) did not cause ultrastructural damage or changes in the cell monolayers. Monoclonal antibody (mAb)-functionalised carbon nanoloops and ssDNA 100% covered Au-nanodots were incorporated and transported within the colon cells using different cellular pathways than those used by the lung cells. Citrate-capped Au-nanoparticles (2 nm and 10 nm) and 20% DNA covered Au-nanoparticles passed into the colon and lung cells through small holes in the apical cell membrane, which could possibly be produced by lipid peroxidation. Serious forms of cell damage were observed with citrate capped 2 nm and 10 nm Au-nanoparticles (i.e., nuclear localisation (2nm-Au); intracellular membrane damage (10 nm-Au)). Vital staining used to identify cellular necrosis following nanoparticle exposure, was sometimes misleading showing cell necrosis statistics similar to normal controls, when TEM analysis revealed intracellular and organellar damage in identically treated cells.

AB - This paper describes some early cellular and intracellular interactions of human polarised lung and colon epithelial cells (representative of two portals of entry, inhalation and ingestion), following exposure to specific carbon and gold engineered nanoparticles in vitro. Cells were incubated with functionalised and non-functionalised carbon nanotube-derived nanoloops (-28-60 nm diameter), or gold nanoparticles (2 nm and 10 nm Au-core) which were either non-functionalised, or functionalised with biological proteins or ssDNA and analysed using viability staining, transmission electron microscopy (TEM) and field emission scanning (FESEM) electron microscopy. Even with such diverse nanoparticles and functionalisations, we found that the surface properties and size of the nanoparticles determined their cellular binding, incorporation and/or cytotoxicity. However the cells responded to the different types of nanoparticles using various intracellular routes which differed with the cell type, but all of the nanoparticles ultimately were consolidated into aggregates and transported to the basal cell surface. Nanoparticles that were completely covered with biological macromolecules (i.e., recombinant gClq-R protein, non-immune IgGk, monoclonal antibody to gClq-R, or ssDNA) did not cause ultrastructural damage or changes in the cell monolayers. Monoclonal antibody (mAb)-functionalised carbon nanoloops and ssDNA 100% covered Au-nanodots were incorporated and transported within the colon cells using different cellular pathways than those used by the lung cells. Citrate-capped Au-nanoparticles (2 nm and 10 nm) and 20% DNA covered Au-nanoparticles passed into the colon and lung cells through small holes in the apical cell membrane, which could possibly be produced by lipid peroxidation. Serious forms of cell damage were observed with citrate capped 2 nm and 10 nm Au-nanoparticles (i.e., nuclear localisation (2nm-Au); intracellular membrane damage (10 nm-Au)). Vital staining used to identify cellular necrosis following nanoparticle exposure, was sometimes misleading showing cell necrosis statistics similar to normal controls, when TEM analysis revealed intracellular and organellar damage in identically treated cells.

KW - Antibody-functionalised

KW - Carbon nanoparticles

KW - Cellular processing

KW - Cytotoxicity

KW - DNA-functionalised

KW - Gold nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=38149038102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38149038102&partnerID=8YFLogxK

U2 - 10.1504/IJNT.2008.016549

DO - 10.1504/IJNT.2008.016549

M3 - Article

AN - SCOPUS:38149038102

VL - 5

SP - 55

EP - 91

JO - International Journal of Nanotechnology

JF - International Journal of Nanotechnology

SN - 1475-7435

IS - 1

ER -