Histone methylation as a marker of chondrocyte phenotype

J. Wang, S. L. Kummer, M. S. Cosgrove, J. H. Henderson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Maintaining articular chondrocyte phenotype is important in terms of pathological conditions and cartilage development, but is complicated and challenging. Epigenetic modifications are one kind of those factors which have effect on cell phenotype regulation. However, the extent to which epigenetics marks in forms of trimethylation of histone H3 Lysine 4 and 27 (H3K4me3 and H3K27me3) regulate chondrocytes phenotype and characteristic gene expression remains largely unknown. In this study, we studied H3K4me3 and H3K27me3 level in the Sox9 promoter in human articular chondrocytes (hACs) comparing human adipose-derived mesenchymal stem cells (hASCs), and global H3K4me3 level in hACs comparing dedifferentiated hACs. Our ChIP-qPCR data showed largely lower H3K27me3 in the Sox9 promoter in hACs than in hASCs, suggesting that repressive chromatin in the Sox9 promoter decreases in the chondrocyte phenotype. Western blotting data showed higher H3K4me3 level in hACs during dedifferentiation. In summary, our results suggest that these epigenetic marks may regulate chondrocyte phenotype, and could be used as unique marks distinguish the hACs phenotype from other lineages and change during dedifferentiation.

Original languageEnglish (US)
Title of host publication2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012
Pages193-194
Number of pages2
DOIs
StatePublished - Jun 29 2012
Event38th Annual Northeast Bioengineering Conference, NEBEC 2012 - Philadelphia, PA, United States
Duration: Mar 16 2012Mar 18 2012

Publication series

Name2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012

Other

Other38th Annual Northeast Bioengineering Conference, NEBEC 2012
CountryUnited States
CityPhiladelphia, PA
Period3/16/123/18/12

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Wang, J., Kummer, S. L., Cosgrove, M. S., & Henderson, J. H. (2012). Histone methylation as a marker of chondrocyte phenotype. In 2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012 (pp. 193-194). [6207029] (2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012). https://doi.org/10.1109/NEBC.2012.6207029