Heme-associated subunit complex of cytochrome c oxidase identified by a new two-dimensional gel electrophoresis.

J. A. Freedman, R. P. Tracy, S. H. Chan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A Novel two-dimensional system (isoelectric focusing in the first dimension and sodium dodecyl sulfate-urea electrophoresis in the second) was used to determine the pI values of beef heart subunits of cytochrome c oxidase. The results identified a heme-associated complex of Subunits I, II, and IV. Conditions mitigate against migration of free heme.

Original languageEnglish (US)
Pages (from-to)4305-4308
Number of pages4
JournalJournal of Biological Chemistry
Volume254
Issue number11
StatePublished - Jun 10 1979
Externally publishedYes

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Electrophoresis, Gel, Two-Dimensional
Electron Transport Complex IV
Electrophoresis
Heme
Gels
Beef
Isoelectric Focusing
Sodium Dodecyl Sulfate
Urea
Red Meat

ASJC Scopus subject areas

  • Biochemistry

Cite this

Heme-associated subunit complex of cytochrome c oxidase identified by a new two-dimensional gel electrophoresis. / Freedman, J. A.; Tracy, R. P.; Chan, S. H.

In: Journal of Biological Chemistry, Vol. 254, No. 11, 10.06.1979, p. 4305-4308.

Research output: Contribution to journalArticle

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