GWAS analysis of QTL for enteric septicemia of catfish and their involved genes suggest evolutionary conservation of a molecular mechanism of disease resistance

Tao Zhou, Shikai Liu, Xin Geng, Yulin Jin, Chen Jiang, Lisui Bao, Jun Yao, Yu Zhang, Jiaren Zhang, Luyang Sun, Xiaozhu Wang, Ning Li, Suxu Tan, Zhanjian "John" Liu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Disease problems cause major economic losses for the aquaculture industries. In catfish, enteric septicemia of catfish (ESC), caused by the bacterial pathogen Edwardsiella ictaluri, is the leading disease problem, causing tens of millions of dollars of annual economic losses. In this study, we conducted a genome-wide association study to determine quantitative trait loci (QTL) for resistance against ESC using an interspecific hybrid system. Five hundred fish were used in the analysis and 192 phenotypic extremes were used for genotyping with the catfish 250K SNP arrays. A genomic region on linkage group (LG) 1 was found significantly associated with ESC disease resistance. In addition, two suggestively associated QTL for ESC resistance were identified on LG 12 and LG 16. The nlrc3 duplicates were identified within all the three QTL, suggesting their importance in association with the QTL. Within the significant QTL on LG 1, 16 genes with known functions in immunity were identified. Of particular interest is the nck1 gene nearby the most significantly associated SNP. Nck1 was known to function as an adaptor to facilitating the pathogenesis of enteropathogenic Escherichia coli (EPEC) in humans. E. ictaluri and EPEC pathogens belong to the same bacterial family and share many common characteristics. The fact that nck1 is mapped in the QTL and that it was significantly upregulated in channel catfish intestine after ESC challenge suggested its candidacy of being involved in resistance/susceptibility of ESC.

Original languageEnglish (US)
Pages (from-to)231-242
Number of pages12
JournalMolecular Genetics and Genomics
Volume292
Issue number1
DOIs
StatePublished - Feb 1 2017
Externally publishedYes

Fingerprint

Catfishes
Disease Resistance
Quantitative Trait Loci
Genome-Wide Association Study
Sepsis
Genes
Edwardsiella ictaluri
Enteropathogenic Escherichia coli
Single Nucleotide Polymorphism
Economics
Ictaluridae
Aquaculture
Intestines
Immunity
Industry
Fishes

Keywords

  • Disease resistance
  • ESC
  • Fish
  • Genome
  • GWAS
  • QTL

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

GWAS analysis of QTL for enteric septicemia of catfish and their involved genes suggest evolutionary conservation of a molecular mechanism of disease resistance. / Zhou, Tao; Liu, Shikai; Geng, Xin; Jin, Yulin; Jiang, Chen; Bao, Lisui; Yao, Jun; Zhang, Yu; Zhang, Jiaren; Sun, Luyang; Wang, Xiaozhu; Li, Ning; Tan, Suxu; Liu, Zhanjian "John".

In: Molecular Genetics and Genomics, Vol. 292, No. 1, 01.02.2017, p. 231-242.

Research output: Contribution to journalArticle

Zhou, Tao ; Liu, Shikai ; Geng, Xin ; Jin, Yulin ; Jiang, Chen ; Bao, Lisui ; Yao, Jun ; Zhang, Yu ; Zhang, Jiaren ; Sun, Luyang ; Wang, Xiaozhu ; Li, Ning ; Tan, Suxu ; Liu, Zhanjian "John". / GWAS analysis of QTL for enteric septicemia of catfish and their involved genes suggest evolutionary conservation of a molecular mechanism of disease resistance. In: Molecular Genetics and Genomics. 2017 ; Vol. 292, No. 1. pp. 231-242.
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