Abstract
Interleukin-1β (IL-1β) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. In spite of its importance and two decades of studies in the mammalian species, genes encoding IL-1β were not identified from non-mammalian species until recently. Recent research, particularly with genomic approaches, has led to sequencing of IL-1β from many species. Clinical studies also suggested IL-1β as an immunoregulatory molecule potentially useful for enhancing vaccination. However, no IL-1β genes have been identified from channel catfish, the primary aquaculture species from the United States. In this study, we identified two distinct cDNAs encoding catfish IL-1β. Their encoding genes were identified, sequenced, and characterized. The catfish IL-1β genes were assigned to bacterial artificial chromosome (BAC) clones. Genomic studies indicated that the IL-1β genes were tandemly duplicated on the same chromosome. Phylogenetic analysis of various IL-1β genes indicated the possibility of recent species-specific gene duplications in channel catfish, and perhaps also in swine and carp. Expression analysis indicated that both IL-1β genes were expressed, but exhibited distinct expression profiles in various catfish tissues, and after bacterial infection with Edwardsiella ictaluri.
Original language | English (US) |
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Pages (from-to) | 1653-1664 |
Number of pages | 12 |
Journal | Molecular Immunology |
Volume | 43 |
Issue number | 10 |
DOIs | |
State | Published - Apr 2006 |
Externally published | Yes |
Keywords
- Catfish
- Fish
- Gene
- IL-1
- Immunity
- Infection
- Inflammation
- Interleukin
ASJC Scopus subject areas
- Immunology
- Molecular Biology