Genomic organization, gene duplication, and expression analysis of interleukin-1β in channel catfish (Ictalurus punctatus)

Yaping Wang, Qun Wang, Puttharat Baoprasertkul, Eric Peatman, Zhanjiang Liu

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Interleukin-1β (IL-1β) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. In spite of its importance and two decades of studies in the mammalian species, genes encoding IL-1β were not identified from non-mammalian species until recently. Recent research, particularly with genomic approaches, has led to sequencing of IL-1β from many species. Clinical studies also suggested IL-1β as an immunoregulatory molecule potentially useful for enhancing vaccination. However, no IL-1β genes have been identified from channel catfish, the primary aquaculture species from the United States. In this study, we identified two distinct cDNAs encoding catfish IL-1β. Their encoding genes were identified, sequenced, and characterized. The catfish IL-1β genes were assigned to bacterial artificial chromosome (BAC) clones. Genomic studies indicated that the IL-1β genes were tandemly duplicated on the same chromosome. Phylogenetic analysis of various IL-1β genes indicated the possibility of recent species-specific gene duplications in channel catfish, and perhaps also in swine and carp. Expression analysis indicated that both IL-1β genes were expressed, but exhibited distinct expression profiles in various catfish tissues, and after bacterial infection with Edwardsiella ictaluri.

Original languageEnglish (US)
Pages (from-to)1653-1664
Number of pages12
JournalMolecular Immunology
Volume43
Issue number10
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

Keywords

  • Catfish
  • Fish
  • Gene
  • IL-1
  • Immunity
  • Infection
  • Inflammation
  • Interleukin

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Genomic organization, gene duplication, and expression analysis of interleukin-1β in channel catfish (Ictalurus punctatus)'. Together they form a unique fingerprint.

  • Cite this