TY - JOUR
T1 - Genomic organization, gene duplication, and expression analysis of interleukin-1β in channel catfish (Ictalurus punctatus)
AU - Wang, Yaping
AU - Wang, Qun
AU - Baoprasertkul, Puttharat
AU - Peatman, Eric
AU - Liu, Zhanjiang
N1 - Funding Information:
This project was supported by a grant from USDA NRI Animal Genome Basic Genome Reagents and Tools Program (USDA/NRICGP 2003-35205-12827). We appreciate the support of Auburn University AAES Foundation Grant and Biogrant from the Office of the Vice President for Research for initiation of the physical mapping project in catfish.
PY - 2006/4
Y1 - 2006/4
N2 - Interleukin-1β (IL-1β) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. In spite of its importance and two decades of studies in the mammalian species, genes encoding IL-1β were not identified from non-mammalian species until recently. Recent research, particularly with genomic approaches, has led to sequencing of IL-1β from many species. Clinical studies also suggested IL-1β as an immunoregulatory molecule potentially useful for enhancing vaccination. However, no IL-1β genes have been identified from channel catfish, the primary aquaculture species from the United States. In this study, we identified two distinct cDNAs encoding catfish IL-1β. Their encoding genes were identified, sequenced, and characterized. The catfish IL-1β genes were assigned to bacterial artificial chromosome (BAC) clones. Genomic studies indicated that the IL-1β genes were tandemly duplicated on the same chromosome. Phylogenetic analysis of various IL-1β genes indicated the possibility of recent species-specific gene duplications in channel catfish, and perhaps also in swine and carp. Expression analysis indicated that both IL-1β genes were expressed, but exhibited distinct expression profiles in various catfish tissues, and after bacterial infection with Edwardsiella ictaluri.
AB - Interleukin-1β (IL-1β) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. In spite of its importance and two decades of studies in the mammalian species, genes encoding IL-1β were not identified from non-mammalian species until recently. Recent research, particularly with genomic approaches, has led to sequencing of IL-1β from many species. Clinical studies also suggested IL-1β as an immunoregulatory molecule potentially useful for enhancing vaccination. However, no IL-1β genes have been identified from channel catfish, the primary aquaculture species from the United States. In this study, we identified two distinct cDNAs encoding catfish IL-1β. Their encoding genes were identified, sequenced, and characterized. The catfish IL-1β genes were assigned to bacterial artificial chromosome (BAC) clones. Genomic studies indicated that the IL-1β genes were tandemly duplicated on the same chromosome. Phylogenetic analysis of various IL-1β genes indicated the possibility of recent species-specific gene duplications in channel catfish, and perhaps also in swine and carp. Expression analysis indicated that both IL-1β genes were expressed, but exhibited distinct expression profiles in various catfish tissues, and after bacterial infection with Edwardsiella ictaluri.
KW - Catfish
KW - Fish
KW - Gene
KW - IL-1
KW - Immunity
KW - Infection
KW - Inflammation
KW - Interleukin
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U2 - 10.1016/j.molimm.2005.09.024
DO - 10.1016/j.molimm.2005.09.024
M3 - Article
C2 - 16280165
AN - SCOPUS:33644500728
SN - 0161-5890
VL - 43
SP - 1653
EP - 1664
JO - Molecular Immunology
JF - Molecular Immunology
IS - 10
ER -