Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection

Lin Song, Chao Li, Yangjie Xie, Shikai Liu, Jiaren Zhang, Jun Yao, Chen Jiang, Yun Li, Zhanjian "John" Liu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Heat shock proteins 70/110 (Hsp70/110) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. Besides the chaperone and housekeeping functions, they are also known to be involved in immune response during infection. In this study, we identified 16 Hsp70/110 geness in channel catfish ( Ictalurus punctatus) through in silico analysis using RNA-Seq and genome databases. Among them 12 members of Hsp70 (Hspa) family and 4 members of Hsp110 (Hsph) family were identified. Phylogenetic and syntenic analyses provided strong evidence in supporting the orthologies of these HSPs. In addition, we also determined the expression patterns of Hsp70/110 genes after Flavobacterium columnare and Edwardsiella ictaluri infections by meta-analyses, for the first time in channel catfish. Ten out of sixteen genes were significantly up/down-regulated after bacterial challenges. Specifically, nine genes were found significantly expressed in gill after F. columnare infection. Two genes were found significantly expressed in intestine after E. ictaluri infection. Pathogen-specific pattern and tissue-specific pattern were found in the two infections. The significantly regulated expressions of catfish Hsp70 genes after bacterial infections suggested their involvement in immune response in catfish.

Original languageEnglish (US)
Pages (from-to)154-162
Number of pages9
JournalFish and Shellfish Immunology
Volume49
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

Ictalurus punctatus
bacterial infections
genome
Genes
heat shock
HSP70 Heat-Shock Proteins
gene
Flavobacterium columnare
Edwardsiella ictaluri
infection
protein
genes
catfish
immune response
heat shock proteins
Pathogens
Heat-Shock Proteins
gills
intestines
RNA

Keywords

  • Catfish
  • Genome
  • Heat shock protein
  • Hsp70
  • Immunity
  • Infection

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

Cite this

Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection. / Song, Lin; Li, Chao; Xie, Yangjie; Liu, Shikai; Zhang, Jiaren; Yao, Jun; Jiang, Chen; Li, Yun; Liu, Zhanjian "John".

In: Fish and Shellfish Immunology, Vol. 49, 01.02.2016, p. 154-162.

Research output: Contribution to journalArticle

Song, Lin ; Li, Chao ; Xie, Yangjie ; Liu, Shikai ; Zhang, Jiaren ; Yao, Jun ; Jiang, Chen ; Li, Yun ; Liu, Zhanjian "John". / Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection. In: Fish and Shellfish Immunology. 2016 ; Vol. 49. pp. 154-162.
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