@article{ee95e54f2162448d8e9d18e6040a63d7,
title = "Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-Triggered hierarchical self-Assembly",
abstract = "Post-Translational modification of proteins is a strategy widely used in biological systems. It expands the diversity of the proteome and allows for tailoring of both the function and localization of proteins within cells as well as the material properties of structural proteins and matrices. Despite their ubiquity in biology, with a few exceptions, the potential of post-Translational modifications in biomaterials synthesis has remained largely untapped. As a proof of concept to demonstrate the feasibility of creating a genetically encoded biohybrid material through post-Translational modification, we report here the generation of a family of three stimulus-responsive hybrid materials-fatty-Acid-modified elastin-like polypeptides-using a one-pot recombinant expression and post-Translational lipidation methodology. These hybrid biomaterials contain an amphiphilic domain, composed of a β-sheet-forming peptide that is post-Translationally functionalized with a C14 alkyl chain, fused to a thermally responsive elastin-like polypeptide. They exhibit temperature-Triggered hierarchical self-Assembly across multiple length scales with varied structure and material properties that can be controlled at the sequence level.",
author = "Davoud Mozhdehi and Luginbuhl, {Kelli M.} and Simon, {Joseph R.} and Michael Dzuricky and R{\"u}diger Berger and Varol, {H. Samet} and Huang, {Fred C.} and Buehne, {Kristen L.} and Mayne, {Nicholas R.} and Isaac Weitzhandler and Mischa Bonn and Parekh, {Sapun H.} and Ashutosh Chilkoti",
note = "Funding Information: This research was funded by the National Science Foundation (NSF) through the Research Triangle Materials Research Science and Engineering Center (MRSEC; DMR-1121107) and by the National Institutes of Health (NIH; R01 GM-061232). Duke University Shared Materials Instrumentation Facility (SMIF) and Analytical Instrumentation facility (AIF) at North Carolina State University are members of the North Carolina Research Triangle NanotechnologyNetwork (RTNN), which is supported by the NSF (ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). The authors thank K. Franz for access to chromatography instrumentation and the peptide synthesizer, J.G. Mark for conducting SDCLM experiments and M. Plue for the SEM imaging. The authors also thank H. Burg for her support in SFM sample preparation and analysis, M.-J. van Zadel for assistance with the variable-temperature ATR-IR experiments and M. Rubinstein for suggestions regarding the mechanism of self-assembly. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = may,
day = "1",
doi = "10.1038/s41557-018-0005-z",
language = "English (US)",
volume = "10",
pages = "496--505",
journal = "Nature Chemistry",
issn = "1755-4330",
publisher = "Nature Publishing Group",
number = "5",
}