Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-Triggered hierarchical self-Assembly

Davoud Mozhdehi, Kelli M. Luginbuhl, Joseph R. Simon, Michael Dzuricky, Rüdiger Berger, H. Samet Varol, Fred C. Huang, Kristen L. Buehne, Nicholas R. Mayne, Isaac Weitzhandler, Mischa Bonn, Sapun H. Parekh, Ashutosh Chilkoti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Post-Translational modification of proteins is a strategy widely used in biological systems. It expands the diversity of the proteome and allows for tailoring of both the function and localization of proteins within cells as well as the material properties of structural proteins and matrices. Despite their ubiquity in biology, with a few exceptions, the potential of post-Translational modifications in biomaterials synthesis has remained largely untapped. As a proof of concept to demonstrate the feasibility of creating a genetically encoded biohybrid material through post-Translational modification, we report here the generation of a family of three stimulus-responsive hybrid materials-fatty-Acid-modified elastin-like polypeptides-using a one-pot recombinant expression and post-Translational lipidation methodology. These hybrid biomaterials contain an amphiphilic domain, composed of a β-sheet-forming peptide that is post-Translationally functionalized with a C14 alkyl chain, fused to a thermally responsive elastin-like polypeptide. They exhibit temperature-Triggered hierarchical self-Assembly across multiple length scales with varied structure and material properties that can be controlled at the sequence level.

Original languageEnglish (US)
Pages (from-to)496-505
Number of pages10
JournalNature Chemistry
Volume10
Issue number5
DOIs
StatePublished - May 1 2018
Externally publishedYes

Fingerprint

Polypeptides
Biocompatible Materials
Biomaterials
Self assembly
Lipids
Elastin
Proteins
Peptides
Materials properties
Hybrid materials
Biological systems
Proteome
Temperature
Fatty Acids
Fatty acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-Triggered hierarchical self-Assembly. / Mozhdehi, Davoud; Luginbuhl, Kelli M.; Simon, Joseph R.; Dzuricky, Michael; Berger, Rüdiger; Varol, H. Samet; Huang, Fred C.; Buehne, Kristen L.; Mayne, Nicholas R.; Weitzhandler, Isaac; Bonn, Mischa; Parekh, Sapun H.; Chilkoti, Ashutosh.

In: Nature Chemistry, Vol. 10, No. 5, 01.05.2018, p. 496-505.

Research output: Contribution to journalArticle

Mozhdehi, D, Luginbuhl, KM, Simon, JR, Dzuricky, M, Berger, R, Varol, HS, Huang, FC, Buehne, KL, Mayne, NR, Weitzhandler, I, Bonn, M, Parekh, SH & Chilkoti, A 2018, 'Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-Triggered hierarchical self-Assembly', Nature Chemistry, vol. 10, no. 5, pp. 496-505. https://doi.org/10.1038/s41557-018-0005-z
Mozhdehi, Davoud ; Luginbuhl, Kelli M. ; Simon, Joseph R. ; Dzuricky, Michael ; Berger, Rüdiger ; Varol, H. Samet ; Huang, Fred C. ; Buehne, Kristen L. ; Mayne, Nicholas R. ; Weitzhandler, Isaac ; Bonn, Mischa ; Parekh, Sapun H. ; Chilkoti, Ashutosh. / Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-Triggered hierarchical self-Assembly. In: Nature Chemistry. 2018 ; Vol. 10, No. 5. pp. 496-505.
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