@article{e85233efbf4148d9ae385c23280901e1,
title = "Genetically Encoded Cholesterol-Modified Polypeptides",
abstract = "Biological systems use post-translational modifications (PTMs) to control the structure, location, and function of proteins after expression. Despite the ubiquity of PTMs in biology, their use to create genetically encoded recombinant biomaterials is limited. We have utilized a natural lipidation PTM (hedgehog-mediated cholesterol modification of proteins) to create a class of hybrid biomaterials called cholesterol-modified polypeptides (CHaMPs) that exhibit programmable self-assembly at the nanoscale. To demonstrate the biomedical utility of CHaMPs, we used this approach to append cholesterol to biologically active peptide exendin-4 that is an approved drug for the treatment of type II diabetes. The exendin-cholesterol conjugate self-assembled into micelles, and these micelles activate the glucagon-like peptide-1 receptor with a potency comparable to that of current gold standard treatments.",
author = "Davoud Mozhdehi and Luginbuhl, {Kelli M.} and Michael Dzuricky and Costa, {Simone A.} and Sinan Xiong and Huang, {Fred C.} and Lewis, {Mae M.} and Zelenetz, {Stephanie R.} and Colby, {Christian D.} and Ashutosh Chilkoti",
note = "Funding Information: The cell line used in this study was a generous gift from Dr. Timothy Kieffer (University of British Columbia). The Duke University Shared Materials Instrumentation Facility (SMIF) is a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Funding Information: *Chilkoti@duke.edu. ORCID Michael Dzuricky: 0000-0002-1775-2132 Ashutosh Chilkoti: 0000-0002-1569-2228 Present Address †Current Address: Department of Chemistry, Syracuse University, Center for Science and Technology, 111 College Place, Syracuse, New York 13244, United States. Funding We acknowledge the financial support of the NSF through NSF MRSEC and DMR-1121107 and the NIH through R01 GM61232 and R35 GM127042. Notes The authors declare no competing financial interest. Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",
year = "2019",
month = jan,
day = "16",
doi = "10.1021/jacs.8b10687",
language = "English (US)",
volume = "141",
pages = "945--951",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "2",
}