Generation of cell-laden hydrogel microspheres using 3D printing-enabled microfluidics

Sanika Suvarnapathaki, Rafael Ramos, Stephen W. Sawyer, Shannon McLoughlin, Andrew Ramos, Sarah Venn, Pranav Soman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

3D printing has been shown to be a robust and inexpensive manufacturing tool for a range of applications within biomedical science. Here we report the design and fabrication of a 3D printer-enabled microfluidic device used to generate cell-laden hydrogel microspheres of tunable sizes. An inverse mold was printed using a 3D printer, and replica molding was used to fabricate a PDMS microfluidic device. Intersecting channel geometry was used to generate perfluorodecalin oil-coated gelatin methacrylate (GelMA) microspheres of varying sizes (35–250 μm diameters). Process parameters such as viscosity profile and UV cross-linking times were determined for a range of GelMA concentrations (7–15% w/v). Empirical relationships between flow rates of GelMA and oil phases, microspheres size, and associated swelling properties were determined. For cell experiments, GelMA was mixed with human osteosarcoma Saos-2 cells, to generate cell-laden GelMA microspheres with high long-term viability. This simple, inexpensive method does not require the use of traditional cleanroom facilities and when combined with the appropriate flow setup is robust enough to yield tunable cell-laden hydrogel microspheres for potential tissue engineering applications.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalJournal of Materials Research
DOIs
StateAccepted/In press - May 15 2018

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Keywords

  • biomaterial
  • cellular (material form)
  • polymer

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Suvarnapathaki, S., Ramos, R., Sawyer, S. W., McLoughlin, S., Ramos, A., Venn, S., & Soman, P. (Accepted/In press). Generation of cell-laden hydrogel microspheres using 3D printing-enabled microfluidics. Journal of Materials Research, 1-7. https://doi.org/10.1557/jmr.2018.77