Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus

Bing Xu; Shaolin Wang; Yan Jiang; Linlin Yang; Ping Li; Caixia Xie; Jubin Xing; Zhonghe Ke; Jiale Li; Junwei Gai; Guimei Yang; Baolong Bao; Zhanjiang Liu

doi:10.1080/10495398.2010.505843

Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus

Bing Xu, Shaolin Wang, Yan Jiang, Linlin Yang, Ping Li, Caixia Xie, Jubin Xing, Zhonghe Ke, Jiale Li, Junwei Gai, Guimei Yang, Baolong Bao, Zhanjiang Liu

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.

Original language	English (US)
Pages (from-to)	217-225
Number of pages	9
Journal	Animal Biotechnology
Volume	21
Issue number	4
DOIs	https://doi.org/10.1080/10495398.2010.505843
State	Published - Oct 2010
Externally published	Yes

Keywords

EST
Genome
Grass carp
Marker
Microsatellite
SNP

ASJC Scopus subject areas

Biotechnology
Bioengineering
Animal Science and Zoology

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10.1080/10495398.2010.505843

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title = "Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus",

abstract = "Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.",

keywords = "EST, Genome, Grass carp, Marker, Microsatellite, SNP",

author = "Bing Xu and Shaolin Wang and Yan Jiang and Linlin Yang and Ping Li and Caixia Xie and Jubin Xing and Zhonghe Ke and Jiale Li and Junwei Gai and Guimei Yang and Baolong Bao and Zhanjiang Liu",

note = "Funding Information: This work was supported by the key foundation program 06dj14003 from Shanghai Municipal Science Committee, China, and partially supported by National Natural Science Foundation of China 30771668, National Education Commission of China 06ZZ65, and the Key Discipline funding Y1101, Marine Biology funding J50701, and Hydrobiology funding S30701.",

year = "2010",

month = oct,

doi = "10.1080/10495398.2010.505843",

language = "English (US)",

volume = "21",

pages = "217--225",

journal = "Animal Biotechnology",

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T1 - Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus

AU - Xu, Bing

AU - Wang, Shaolin

AU - Jiang, Yan

AU - Yang, Linlin

AU - Li, Ping

AU - Xie, Caixia

AU - Xing, Jubin

AU - Ke, Zhonghe

AU - Li, Jiale

AU - Gai, Junwei

AU - Yang, Guimei

AU - Bao, Baolong

AU - Liu, Zhanjiang

N1 - Funding Information: This work was supported by the key foundation program 06dj14003 from Shanghai Municipal Science Committee, China, and partially supported by National Natural Science Foundation of China 30771668, National Education Commission of China 06ZZ65, and the Key Discipline funding Y1101, Marine Biology funding J50701, and Hydrobiology funding S30701.

PY - 2010/10

Y1 - 2010/10

N2 - Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.

AB - Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.

KW - EST

KW - Genome

KW - Grass carp

KW - Marker

KW - Microsatellite

KW - SNP

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Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus

Abstract

Keywords

ASJC Scopus subject areas

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