Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus

Bing Xu, Shaolin Wang, Yan Jiang, Linlin Yang, Ping Li, Caixia Xie, Jubin Xing, Zhonghe Ke, Jiale Li, Junwei Gai, Guimei Yang, Baolong Bao, Zhanjian "John" Liu

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalAnimal Biotechnology
Volume21
Issue number4
DOIs
StatePublished - Oct 2010
Externally publishedYes

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Keywords

  • EST
  • Genome
  • Grass carp
  • Marker
  • Microsatellite
  • SNP

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Animal Science and Zoology

Cite this

Xu, B., Wang, S., Jiang, Y., Yang, L., Li, P., Xie, C., Xing, J., Ke, Z., Li, J., Gai, J., Yang, G., Bao, B., & Liu, Z. J. (2010). Generation and analysis of ESTs from the Grass carp, Ctenopharyngodon idellus. Animal Biotechnology, 21(4), 217-225. https://doi.org/10.1080/10495398.2010.505843