Fluorescence studies of bilayers and proteins: Critical behavior and genetic engineering

Bruce Hudson, Anthony Ruggiero, Danni Harris, Lain Johnson, Xiu Mei Dou, Thomas Novet, Lawrence McIntosh, Cynthia Phillips, Theresa Nester

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Fluorescence decay and anisotropy studies of parinaric acid in phospholipid bilayers have demonstrated the presence of density fluctuations exhibiting critical behavior in these nominally single phase structures. The anisotropy behavior is used to extract the second and fourth rank order parameters <P2> and <P4> for comparison with models of acyl chain order. A recent study of parinaric acid in hexagonal urea inclusion complexes has demonstrated that the strongly allowed transition is polarized at an angle with respect to the chain axis. The implications of this for the interpretation of anisotropy experiments is discussed. The lysozyme from bacteriophage aq has been engineered in modified forms containing only one tryptophan residue and with substitutions near the buried tryptophan residue 138. Simple changes in the structure appear to result in large changes in the dynamics of this residue. These observations are compared with the results of molecular dynamics computations.

Original languageEnglish (US)
Pages (from-to)113-120
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Jun 24 1988
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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