Fluorescence Lifetime and Time-Resolved Polarization Anisotropy Studies of Acyl Chain Order and Dynamics in Lipid Bilayers

Paul K. Wolber, Bruce S. Hudson

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

The time-resolved fluorescence intensity and anisotropy decays of cis- and irtm-parinaric acids and phosphatidylcholines labeled with trans-parinaric acid have been characterized in bilayers formed by several phosphatidylcholines and by dipalmitoylphosphatidylcholine-cholesterol mixtures, at several temperatures. Both a conventional freerunning nitrogen flashlamp and the novel synchrotron source at the Stanford Linear Accelerator Center (SLAC) were used as excitation sources for a modified single photon counting fluorescence lifetime apparatus. The measured emission decay kinetics of both isomers of parinaric acid were biexponential in all but one of the lipid systems examined. The fluorescence anisotropy of parinaric acid was large and constant in gel phase lipids, but showed a very rapid (~2 GHz) decay of large amplitude in fluid lipids. In all lipid systems studied, the fluorescence anisotropy decayed to a nonzero asymptote, in striking contrast to the behavior observed in viscous solvent solutions. The asymptotic anisotropy was used to calculate an “order parameter” of the emission transition dipole. The value of the order parameter is quite close to that obtained by deuterium NMR. Cholesterol increased the order parameter measured in fluid dipalmitoylphosphatidylcholine but did not substantially affect the rate of angular relaxation. Experiments conducted with franj-parinaroylphosphatidylcholines yielded results virtually identical with those obtained with rranj-parinaric acid.

Original languageEnglish (US)
Pages (from-to)2800-2810
Number of pages11
JournalBiochemistry
Volume20
Issue number10
DOIs
StatePublished - May 1981

ASJC Scopus subject areas

  • Biochemistry

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