Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy

Hyeong Gon Kang, Mathew M. Maye, Dmytro Nykypanchuk, Matthew Clarke, Peter Yim, Jeffrey Krogmeier, Kimberly Briggman, Oleg Gang, Jeeseong Hwang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

We have fabricated a combined measurement system capable of confocal microscopy and fluorescence spectroscopy to simultaneously evaluate multiple optical characteristics of single fluorescent nanocrystals. The single particle detection sensitivity is demonstrated by simultaneously measuring the dynamic excitation-time-dependent fluorescence intermittency and the emission spectrum of single cadmium selenide/zinc sulfide (CdSe/ZnS) nanocrystals (quantum dots, QDs). Using this system, we are currently investigating the optical characteristics of single QDs, the surface of which are conjugated with different ligands, such as trioctylphosphine oxide (TOPO), mercaptoundecanoicacid (MDA), and amine modified DNA (AMDNA). In this paper, we present the progress of our measurements of the time-dependent optical characteristics (fluorescence intermittency, photostability, and spectral diffusion) of single MDA-QDs and AMDNA-MDA-QDs in air in an effort to understand the effects of surface-conjugated biomolecules on the optical characteristics at single QD sensitivities.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems V
DOIs
StatePublished - Apr 30 2007
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic Systems V - San Jose, CA, United States
Duration: Jan 21 2007Jan 27 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6430
ISSN (Print)1605-7422

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems V
CountryUnited States
CitySan Jose, CA
Period1/21/071/27/07

Keywords

  • Bio-conjugation
  • Confocal microscopy
  • Nanocrystal
  • Nanosensor
  • Photophysics
  • Quantum dot
  • Single molecule
  • Single particle
  • Spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy'. Together they form a unique fingerprint.

  • Cite this

    Kang, H. G., Maye, M. M., Nykypanchuk, D., Clarke, M., Yim, P., Krogmeier, J., Briggman, K., Gang, O., & Hwang, J. (2007). Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy. In Advanced Biomedical and Clinical Diagnostic Systems V [643024] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6430). https://doi.org/10.1117/12.713934