Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy

HyeongGon Kang, Mathew M Maye, Dmytro Nykypanchuk, Matthew Clarke, Peter Yim, Jeffrey Krogmeier, Kimberly Briggman, Oleg Gang, Jeeseong Hwang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We have fabricated a combined measurement system capable of confocal microscopy and fluorescence spectroscopy to simultaneously evaluate multiple optical characteristics of single fluorescent nanocrystals. The single particle detection sensitivity is demonstrated by simultaneously measuring the dynamic excitation-time-dependent fluorescence intermittency and the emission spectrum of single cadmium selenide/zinc sulfide (CdSe/ZnS) nanocrystals (quantum dots, QDs). Using this system, we are currently investigating the optical characteristics of single QDs, the surface of which are conjugated with different ligands, such as trioctylphosphine oxide (TOPO), mercaptoundecanoicacid (MDA), and amine modified DNA (AMDNA). In this paper, we present the progress of our measurements of the time-dependent optical characteristics (fluorescence intermittency, photostability, and spectral diffusion) of single MDA-QDs and AMDNA-MDA-QDs in air in an effort to understand the effects of surface-conjugated biomolecules on the optical characteristics at single QD sensitivities.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6430
DOIs
StatePublished - 2007
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic Systems V - San Jose, CA, United States
Duration: Jan 21 2007Jan 27 2007

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems V
CountryUnited States
CitySan Jose, CA
Period1/21/071/27/07

Fingerprint

Confocal microscopy
Fluorescence microscopy
Fluorescence spectroscopy
Nanocrystals
Semiconductor quantum dots
Fluorescence
Molecules
Amines
DNA
Zinc sulfide
Biomolecules
Cadmium
Ligands
Oxides
Air

Keywords

  • Bio-conjugation
  • Confocal microscopy
  • Nanocrystal
  • Nanosensor
  • Photophysics
  • Quantum dot
  • Single molecule
  • Single particle
  • Spectroscopy

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kang, H., Maye, M. M., Nykypanchuk, D., Clarke, M., Yim, P., Krogmeier, J., ... Hwang, J. (2007). Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6430). [643024] https://doi.org/10.1117/12.713934

Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy. / Kang, HyeongGon; Maye, Mathew M; Nykypanchuk, Dmytro; Clarke, Matthew; Yim, Peter; Krogmeier, Jeffrey; Briggman, Kimberly; Gang, Oleg; Hwang, Jeeseong.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6430 2007. 643024.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kang, H, Maye, MM, Nykypanchuk, D, Clarke, M, Yim, P, Krogmeier, J, Briggman, K, Gang, O & Hwang, J 2007, Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6430, 643024, Advanced Biomedical and Clinical Diagnostic Systems V, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.713934
Kang H, Maye MM, Nykypanchuk D, Clarke M, Yim P, Krogmeier J et al. Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6430. 2007. 643024 https://doi.org/10.1117/12.713934
Kang, HyeongGon ; Maye, Mathew M ; Nykypanchuk, Dmytro ; Clarke, Matthew ; Yim, Peter ; Krogmeier, Jeffrey ; Briggman, Kimberly ; Gang, Oleg ; Hwang, Jeeseong. / Fluorescence intermittency and spectral shifts of single bioconjugated nanocrystals studied by single molecule confocal fluorescence microscopy and spectroscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6430 2007.
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AU - Yim, Peter

AU - Krogmeier, Jeffrey

AU - Briggman, Kimberly

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