Fluorescence imaging of nanoscale domains in polymer blends using Stochastic Optical Reconstruction Microscopy (STORM)

M. W. Gramlich, J. Bae, R. C. Hayward, J. L. Ross

Research output: Contribution to journalArticle

16 Scopus citations


High-resolution fluorescence techniques that provide spatial resolution below the diffraction limit are attractive new methods for structural characterization of nanostructured materials. For the first time, we apply the super-resolution technique of Stochastic Optical Reconstruction Microscopy (STORM), to characterize nanoscale structures within polymer blend films. The STORM technique involves temporally separating the fluorescence signals from individual labeled polymers, allowing their positions to be localized with high accuracy, yielding a high-resolution composite image of the material. Here, we describe the application of the technique to demixed blend films of polystyrene (PS) and poly(methyl methacrylate) (PMMA), and find that STORM provides comparable structural characteristics as those determined by Atomic Force Microscopy (AFM) and scanning electron microscopy (SEM), but with all of the advantages of a far-field optical technique

Original languageEnglish (US)
Pages (from-to)8438-8450
Number of pages13
JournalOptics Express
Issue number7
StatePublished - Jan 1 2014
Externally publishedYes


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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