Fluorescence Assay for Ghrelin O-Acyltransferase Activity

James Hougland (Inventor), Joseph Darling (Inventor)

Research output: Patent


An assay to detect ghrelin O-acyltransferase activity using an acrylodan-labeled peptide mimic of ghrelin that provides for high-throughput screening for ghrelin O-acyltransferase inhibitors and detection via high performance liquid chromatography. Alternatively, the assay for ghrelin acylation may be based on a synthetic peptide substrate that mimics the N-terminal sequence of ghrelin and has an environmentally-sensitive fluorophore attached to its C-terminal amino acid through chemoselective ligation.
Original languageEnglish (US)
Patent number9,115,383
StatePublished - Jul 31 2014


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