Flow cytometric analysis of DNA content for tropical and temperate new world pines

S. E. Hall, W. S. Dvorak, J. S. Johnston, H. J. Price, C. G. Williams

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Temperate pine species have unusually large, complex genomes which make genomic analysis problematic; it has been suggested that tropical pines might have smaller genome sizes than temperate pines. Laser flow cytometry (LFC) was used to measure genome sizes of 11 species from Mexico, Guatemala and Nicaragua, spanning latitudes 14°-37° N. These values were compared with previously reported LFC estimates for 17 subtropical and temperate species. Genome sizes in this study were large, varying 1·6-fold from 19·94 to 31·76 pg/C. Genome size variation paralleled taxonomic classification more closely than latitudinal origin. Genome sizes of subgenus Strobus (soft pines) were, larger, ranging from 27·36 to 31·76 pg/C; those of subgenus Pinus (hard pines) were smaller, ranging from 19·94 to 24·91 pg/C. The exception was hard pine subsection Macrocarpae which had larger genome sizes ranging from 26·33 to 28·35. Intraspecific variation was substantial for tropical hard pines P. oocarpa and P. tecunumanii yet negligible for tropical hard pine P. patula.

Original languageEnglish (US)
Pages (from-to)1081-1086
Number of pages6
JournalAnnals of Botany
Volume86
Issue number6
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Keywords

  • C-values
  • Conifers
  • Genome size
  • Gymnosperms
  • Latitudinal variation
  • Megagametophytes
  • Pinus spp.

ASJC Scopus subject areas

  • Plant Science

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