Female mouse germ cells form synchronously dividing cysts

Melissa E. Pepling, Allan C. Spradling

Research output: Contribution to journalArticle

262 Scopus citations

Abstract

Oocytes from many invertebrates initiate development within distinctive cysts of interconnected cells, which are formed through synchronous divisions of a progenitor cell. Recently, processes underlying cyst formation have been extensively characterized at the molecular level in Drosophila. Defects in this process cause sterility in female flies. Early female mouse germ cells are organized as cell clusters as well, but it is uncertain whether these groups are similar to the cysts of invertebrates. We find that mouse germ cells are connected by intercellular bridges in the ovaries of 11.5 to 17.5 days postcoitum embryos; microtubules and organelles have been observed within these bridges. Confocal microscopy shows that cells within mouse clusters divide synchronously and frequently correspond in number to powers of two. Thus, female mouse germ cell clusters exhibit key characteristics of invertebrate germline cysts indicating that the process of germline cyst formation is conserved in the mouse.

Original languageEnglish (US)
Pages (from-to)3323-3328
Number of pages6
JournalDevelopment
Volume125
Issue number17
StatePublished - Sep 1998

Keywords

  • Cyst
  • Mouse germ cell
  • Oogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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