Expression of the neurofibromatosis 1 (NF1) gene in reactive astrocytes in vitro

Sandra J. Hewett, Dennis W. Choi, David H. Gutmann

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

NEUROFIBROMIN, the product of the neurofibromatosis 1 (NF1) gene, is an important tumor suppressor protein expressed most abundantly in the nervous system. Within the central nervous system, neurofibromin has been localized to neurons and oligodendrocytes but not astrocytes. As individuals with NF1 are at an increased risk for optic pathway gliomas and astrocytomas, we chose to re-evaluate the level of neurofibromin expression in primary cultures of murine cortical astrocytes under control and reactive conditions. Astrocytes under control conditions expressed low levels of NF1 mRNA and protein. Following stimulation with dibutyryl-cyclic AMP or interferon-γ in combination with either lipopolysaccharide or interleukin-lβ, there was a marked increase in NF1 mRNA and protein expression. These results suggest that neurofibromin may be involved in the process of reactive astrocytosis seen in response to CNS injury.

Original languageEnglish (US)
Pages (from-to)1565-1568
Number of pages4
JournalNeuroReport
Volume6
Issue number11
DOIs
StatePublished - Jul 1995
Externally publishedYes

Keywords

  • Glia
  • Neurofibromin
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Neuroscience(all)

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