TY - JOUR
T1 - Expression of genes encoding Ca2+ exporting proteins in freshwater crayfish Procambarus clarkii during cold exposure
AU - Gao, Yongping
AU - Gillen, Christopher M.
AU - Whalen, Daniel R.
AU - Vigo, Francieli M.
AU - Golshani, Ashkahn E.
AU - Wheatly, Michele G.
N1 - Funding Information:
The authors thank Dr. Steven Berberich in the Center for Genomics Research at Wright State University for expert technical advice in real-time PCR, and Dr. Mariana Morris and Ms. Mary Key in the Pharmacology/Toxicology Department at Wright State University the help with the in situ hybridization. The authors also thank Mr. Laxminath Tumburu and Ms. Yang Gao, graduate students in the Biological Sciences Department of Wright State University, for their assistance with the western blots. Support for this research was provided by National Science Foundation Grant IBN 0076035 to MGW. FV's visit was supported by US-Brazil higher education consortium grant from Department of Education (FIPSE) P116M010019-04.
PY - 2009/4
Y1 - 2009/4
N2 - 1.This study examined expression of two primary transmembrane Ca2+ export proteins (plasma membrane Ca2+ ATPase, (PMCA); Na+/Ca2+ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C).2.Crayfish tissues responded to cold exposure within 5-7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4-5× higher in antennal gland and 2-3× higher in muscle. In situ hybridization localized the mRNA increase to the nephridial canal in antennal gland sections and to the extensor muscles in the tail. Western blotting confirmed corresponding increases in PMCA protein expression; however, expression of NCX protein was unchanged.
AB - 1.This study examined expression of two primary transmembrane Ca2+ export proteins (plasma membrane Ca2+ ATPase, (PMCA); Na+/Ca2+ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C).2.Crayfish tissues responded to cold exposure within 5-7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4-5× higher in antennal gland and 2-3× higher in muscle. In situ hybridization localized the mRNA increase to the nephridial canal in antennal gland sections and to the extensor muscles in the tail. Western blotting confirmed corresponding increases in PMCA protein expression; however, expression of NCX protein was unchanged.
KW - Antennal gland
KW - Axial abdominal muscle
KW - Calcium export
KW - Cold exposure
KW - Crayfish
KW - Plasma membrane calcium ATPase (PMCA)
KW - Procambarus clarkii
KW - Sodium/calcium exchanger (NCX)
UR - http://www.scopus.com/inward/record.url?scp=63049083092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63049083092&partnerID=8YFLogxK
U2 - 10.1016/j.jtherbio.2009.01.002
DO - 10.1016/j.jtherbio.2009.01.002
M3 - Article
AN - SCOPUS:63049083092
VL - 34
SP - 144
EP - 151
JO - Journal of Thermal Biology
JF - Journal of Thermal Biology
SN - 0306-4565
IS - 3
ER -