Expression of genes encoding Ca2+ exporting proteins in freshwater crayfish Procambarus clarkii during cold exposure

Yongping Gao; Christopher M. Gillen; Daniel R. Whalen; Francieli M. Vigo; Ashkahn E. Golshani; Michele G. Wheatly

doi:10.1016/j.jtherbio.2009.01.002

Expression of genes encoding Ca²⁺ exporting proteins in freshwater crayfish Procambarus clarkii during cold exposure

Yongping Gao, Christopher M. Gillen, Daniel R. Whalen, Francieli M. Vigo, Ashkahn E. Golshani, Michele G. Wheatly

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

1.This study examined expression of two primary transmembrane Ca²⁺ export proteins (plasma membrane Ca²⁺ ATPase, (PMCA); Na⁺/Ca²⁺ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C).2.Crayfish tissues responded to cold exposure within 5-7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4-5× higher in antennal gland and 2-3× higher in muscle. In situ hybridization localized the mRNA increase to the nephridial canal in antennal gland sections and to the extensor muscles in the tail. Western blotting confirmed corresponding increases in PMCA protein expression; however, expression of NCX protein was unchanged.

Original language	English (US)
Pages (from-to)	144-151
Number of pages	8
Journal	Journal of Thermal Biology
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/j.jtherbio.2009.01.002
State	Published - Apr 2009
Externally published	Yes

Keywords

Antennal gland
Axial abdominal muscle
Calcium export
Cold exposure
Crayfish
Plasma membrane calcium ATPase (PMCA)
Procambarus clarkii
Sodium/calcium exchanger (NCX)

ASJC Scopus subject areas

Biochemistry
Physiology
Agricultural and Biological Sciences(all)
Developmental Biology

Access to Document

10.1016/j.jtherbio.2009.01.002

Cite this

@article{24db47d607d94adcb57ee6bd454537b5,

title = "Expression of genes encoding Ca2+ exporting proteins in freshwater crayfish Procambarus clarkii during cold exposure",

abstract = "1.This study examined expression of two primary transmembrane Ca2+ export proteins (plasma membrane Ca2+ ATPase, (PMCA); Na+/Ca2+ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C).2.Crayfish tissues responded to cold exposure within 5-7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4-5× higher in antennal gland and 2-3× higher in muscle. In situ hybridization localized the mRNA increase to the nephridial canal in antennal gland sections and to the extensor muscles in the tail. Western blotting confirmed corresponding increases in PMCA protein expression; however, expression of NCX protein was unchanged.",

keywords = "Antennal gland, Axial abdominal muscle, Calcium export, Cold exposure, Crayfish, Plasma membrane calcium ATPase (PMCA), Procambarus clarkii, Sodium/calcium exchanger (NCX)",

author = "Yongping Gao and Gillen, {Christopher M.} and Whalen, {Daniel R.} and Vigo, {Francieli M.} and Golshani, {Ashkahn E.} and Wheatly, {Michele G.}",

note = "Funding Information: The authors thank Dr. Steven Berberich in the Center for Genomics Research at Wright State University for expert technical advice in real-time PCR, and Dr. Mariana Morris and Ms. Mary Key in the Pharmacology/Toxicology Department at Wright State University the help with the in situ hybridization. The authors also thank Mr. Laxminath Tumburu and Ms. Yang Gao, graduate students in the Biological Sciences Department of Wright State University, for their assistance with the western blots. Support for this research was provided by National Science Foundation Grant IBN 0076035 to MGW. FV's visit was supported by US-Brazil higher education consortium grant from Department of Education (FIPSE) P116M010019-04.",

year = "2009",

month = apr,

doi = "10.1016/j.jtherbio.2009.01.002",

language = "English (US)",

volume = "34",

pages = "144--151",

journal = "Journal of Thermal Biology",

issn = "0306-4565",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Expression of genes encoding Ca2+ exporting proteins in freshwater crayfish Procambarus clarkii during cold exposure

AU - Gao, Yongping

AU - Gillen, Christopher M.

AU - Whalen, Daniel R.

AU - Vigo, Francieli M.

AU - Golshani, Ashkahn E.

AU - Wheatly, Michele G.

N1 - Funding Information: The authors thank Dr. Steven Berberich in the Center for Genomics Research at Wright State University for expert technical advice in real-time PCR, and Dr. Mariana Morris and Ms. Mary Key in the Pharmacology/Toxicology Department at Wright State University the help with the in situ hybridization. The authors also thank Mr. Laxminath Tumburu and Ms. Yang Gao, graduate students in the Biological Sciences Department of Wright State University, for their assistance with the western blots. Support for this research was provided by National Science Foundation Grant IBN 0076035 to MGW. FV's visit was supported by US-Brazil higher education consortium grant from Department of Education (FIPSE) P116M010019-04.

PY - 2009/4

Y1 - 2009/4

N2 - 1.This study examined expression of two primary transmembrane Ca2+ export proteins (plasma membrane Ca2+ ATPase, (PMCA); Na+/Ca2+ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C).2.Crayfish tissues responded to cold exposure within 5-7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4-5× higher in antennal gland and 2-3× higher in muscle. In situ hybridization localized the mRNA increase to the nephridial canal in antennal gland sections and to the extensor muscles in the tail. Western blotting confirmed corresponding increases in PMCA protein expression; however, expression of NCX protein was unchanged.

AB - 1.This study examined expression of two primary transmembrane Ca2+ export proteins (plasma membrane Ca2+ ATPase, (PMCA); Na+/Ca2+ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C).2.Crayfish tissues responded to cold exposure within 5-7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4-5× higher in antennal gland and 2-3× higher in muscle. In situ hybridization localized the mRNA increase to the nephridial canal in antennal gland sections and to the extensor muscles in the tail. Western blotting confirmed corresponding increases in PMCA protein expression; however, expression of NCX protein was unchanged.

KW - Antennal gland

KW - Axial abdominal muscle

KW - Calcium export

KW - Cold exposure

KW - Crayfish

KW - Plasma membrane calcium ATPase (PMCA)

KW - Procambarus clarkii

KW - Sodium/calcium exchanger (NCX)

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U2 - 10.1016/j.jtherbio.2009.01.002

DO - 10.1016/j.jtherbio.2009.01.002

M3 - Article

AN - SCOPUS:63049083092

SN - 0306-4565

VL - 34

SP - 144

EP - 151

JO - Journal of Thermal Biology

JF - Journal of Thermal Biology

IS - 3

ER -