Expression of Bcl-2 genes in channel catfish after bacterial infection and hypoxia stress

Zihao Yuan, Shikai Liu, Jun Yao, Qifan Zeng, Suxu Tan, Zhanjian "John" Liu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bcl-2 proteins are of vital importance in regulation of apoptosis, and are involved in a number of biological processes such as carcinogenesis and immune responses. Bcl-2 genes have been well studied in mammals, while they are not well investigated in teleost fish including channel catfish, the major aquaculture species in the United States. In this study, we identified 34 bcl-2 genes from the channel catfish genome, and verified their identities by conducting phylogenetic and syntenic analyses. The expression profiles of the bcl-2 genes in response to bacterial infections (Edwardsiella ictaluri and Flavobacterium columnare) and hypoxia stress were determined by performing meta-analysis using the existing RNA-Seq datasets. Differential expressions of bcl-2 genes were observed after bacterial infections and hypoxia treatment, including 22 bcl-2 genes after E. ictaluri infection, 22 bcl-2 genes after F. columnare infection, and 19 bcl-2 genes after hypoxia stress. Overall, the expression of the pro-apoptotic bcl-2 genes were repressed after bacterial infection and hypoxia stress, indicating that bcl-2 genes are potentially involved in the stress response by reducing cell apoptosis. Some bcl-2 genes, such as bcl2b, mcl1a, bmf1, and bnip3, showed different expression pattern during the E. ictaluri and F. columnare infection, suggesting the difference in the pathogenicity of diseases. This work presented the first systematic identification and annotation of bcl-2 genes in catfish, providing essential genomic resources for further immune and physiological studies.

Original languageEnglish (US)
Pages (from-to)79-90
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume65
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Fingerprint

Ictaluridae
bcl-2 Genes
Bacterial Infections
Edwardsiella ictaluri
Hypoxia
Infection
Apoptosis
Flavobacterium
Biological Phenomena
Catfishes
Aquaculture
Virulence
Meta-Analysis
Mammals
Carcinogenesis
Fishes

Keywords

  • Bcl-2
  • Disease
  • Expression
  • Fish
  • Genome
  • Hypoxia

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Expression of Bcl-2 genes in channel catfish after bacterial infection and hypoxia stress. / Yuan, Zihao; Liu, Shikai; Yao, Jun; Zeng, Qifan; Tan, Suxu; Liu, Zhanjian "John".

In: Developmental and Comparative Immunology, Vol. 65, 01.12.2016, p. 79-90.

Research output: Contribution to journalArticle

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