Expression analysis of the acute phase response in channel catfish (Ictalurus punctatus) after infection with a Gram-negative bacterium

Eric Peatman, Puttharat Baoprasertkul, Jeffery Terhune, Peng Xu, Samiran Nandi, Huseyin Kucuktas, Ping Li, Shaolin Wang, Benjaporn Somridhivej, Rex Dunham, Zhanjiang Liu

Research output: Contribution to journalArticle

140 Scopus citations

Abstract

The acute phase response (APR) is a set of metabolic and physiological reactions occurring in the host in response to tissue infection or injury and is a crucial component of the larger innate immune response. The APR is best characterized by dramatic changes in the concentration of a group of plasma proteins known as acute phase proteins (APPs) which are synthesized in the liver and function in a wide range of immunity-related activities. Utilizing a new high-density in situ oligonucleotide microarray, we have evaluated the APR in channel catfish liver following infection with Edwardsiella ictaluri, a bacterial pathogen that causes enteric septicemia of catfish. Our catfish microarray design (28K) builds upon a previous 19K channel catfish array by adding recently sequenced immune transcripts from channel catfish along with 7159 unique sequences from closely related blue catfish. The analysis of microarray results using a traditional 2-fold change in gene expression cutoff and a 10% false-discovery rate revealed a well-developed APR in catfish, with particularly high upregulation (>50-fold) of genes involved in iron homeostasis (i.e. intelectin, hemopexin, haptoglobin, ferritin, and transferrin). Other classical APP genes upregulated greater than 2-fold included coagulation factors, proteinase inhibitors, transport proteins, and complement components. Upregulation of the majority of the complement cascade was observed including the membrane attack complex components and complement inhibitors. A number of pathogen recognition receptors (PRRs) and chemokines were also differentially expressed in the liver following infection. Independent testing of a selection of differentially expressed genes with real-time RT-PCR confirmed microarray results.

Original languageEnglish (US)
Pages (from-to)1183-1196
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume31
Issue number11
DOIs
StatePublished - Oct 24 2007
Externally publishedYes

Keywords

  • Acute phase response
  • Catfish
  • Fish
  • Immunity
  • Infection
  • Microarray

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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    Peatman, E., Baoprasertkul, P., Terhune, J., Xu, P., Nandi, S., Kucuktas, H., Li, P., Wang, S., Somridhivej, B., Dunham, R., & Liu, Z. (2007). Expression analysis of the acute phase response in channel catfish (Ictalurus punctatus) after infection with a Gram-negative bacterium. Developmental and Comparative Immunology, 31(11), 1183-1196. https://doi.org/10.1016/j.dci.2007.03.003