Ethidium analogues with improved resolution in the dye-buoyant density procedure

Bruce Hudson; John H. Dawson; Russell Desiderio; Carol W. Mosher

doi:10.1093/nar/4.5.1349

Ethidium analogues with improved resolution in the dye-buoyant density procedure

Bruce Hudson, John H. Dawson, Russell Desiderio, Carol W. Mosher

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.

Original language	English (US)
Pages (from-to)	1349-1360
Number of pages	12
Journal	Nucleic acids research
Volume	4
Issue number	5
DOIs	https://doi.org/10.1093/nar/4.5.1349
State	Published - Jun 1977
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1093/nar/4.5.1349

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@article{74f3b70b95cf4c278f8d84e4abbe0ae4,

title = "Ethidium analogues with improved resolution in the dye-buoyant density procedure",

abstract = "Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.",

author = "Bruce Hudson and Dawson, {John H.} and Russell Desiderio and Mosher, {Carol W.}",

note = "Funding Information: ACKNOWLEDGMENTS This work was supported by a grant from the Research Corporation and U. S. Public Health Service grant CA15461. B. H. is a Camille and Henry Dreyfus Teacher-Scholar and an Alfred P. Sloan Foundation Fellow. We wish to thank Eric Rothenberg who prepared the PM2 phage. J.D.'s present address is Department of Chemistry, California Institute of Technology, Pasadena, California 91125.",

year = "1977",

month = jun,

doi = "10.1093/nar/4.5.1349",

language = "English (US)",

volume = "4",

pages = "1349--1360",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - Ethidium analogues with improved resolution in the dye-buoyant density procedure

AU - Hudson, Bruce

AU - Dawson, John H.

AU - Desiderio, Russell

AU - Mosher, Carol W.

N1 - Funding Information: ACKNOWLEDGMENTS This work was supported by a grant from the Research Corporation and U. S. Public Health Service grant CA15461. B. H. is a Camille and Henry Dreyfus Teacher-Scholar and an Alfred P. Sloan Foundation Fellow. We wish to thank Eric Rothenberg who prepared the PM2 phage. J.D.'s present address is Department of Chemistry, California Institute of Technology, Pasadena, California 91125.

PY - 1977/6

Y1 - 1977/6

N2 - Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.

AB - Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.

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VL - 4

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EP - 1360

JO - Nucleic acids research

JF - Nucleic acids research

IS - 5

ER -

Ethidium analogues with improved resolution in the dye-buoyant density procedure

Abstract

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