Epithelial calcium transport in crustaceans: Adaptation to intrinsic and extrinsic stressors

Michele Wheatly, Yongping Gao, Christopher M. Gillen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Since the classical studies of Ussing employing a nonmammalian isolated epithelium (frog skin) to explore the basic principles of ion transport, physiologists have adopted increasingly reductionist approaches to dissect the biophysical mechanisms undergirding biological transport. In vitro characterization has employed isolated perfused organs, isolated epithelia, and reconstituted vesicle studies. Depth of resolution has been further enhanced by the emerging molecular revolution. Following years of deconstruction, physiologists are now engaging in reconstruction, namely putting the genes back into the organism. This contribution attempts such an integrative approach for a single electrolyte, calcium (Ca2+), in a nonmammalian epithelium, the crayfish antennal gland (kidney). Two collaborating laboratories have archived an inventory of Ca2+ associated proteins believed to play a role in transcellular Ca2+ movement. Using the basic building blocks (expression profiles of key Ca2+ associated proteins and their regulators), the authors attempt to reconstruct a whole cell model for Ca2+ regulation in transporting epithelium (compared with a nonepithelial tissue) under stressors that perturb Ca2+ homeostasis which originate either intrinsically (the postmolt stage of the molting cycle) or extrinsically (unanticipated cold acclimation). Through horizontal integration of expression profiles of seven target Ca2+ associated proteins in epithelial and nonepithelial tissue under two contrasting experimental conditions, emergent themes inform the physiological complexity of Ca2+ homeostasis. Integration at the next level will require placing the epithelium in the context of organismic Ca2+ balance. The unique Ca2+ handling capabilities of the freshwater crayfish make it an excellent nonmammalian model for those studies.

Original languageEnglish (US)
Title of host publicationEpithelial Transport Physiology
PublisherHumana Press Inc.
Pages73-90
Number of pages18
ISBN (Electronic)9781603272292
ISBN (Print)9781603272285
DOIs
StatePublished - 2010
Externally publishedYes

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Keywords

  • Antennal gland
  • Calcium
  • CaM
  • Cold
  • Crayfish
  • ECaC
  • eEF1Bγ
  • Muscle
  • NCX
  • PMCA
  • Posmolt
  • SCP
  • SERCA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Wheatly, M., Gao, Y., & Gillen, C. M. (2010). Epithelial calcium transport in crustaceans: Adaptation to intrinsic and extrinsic stressors. In Epithelial Transport Physiology (pp. 73-90). Humana Press Inc.. https://doi.org/10.1007/978-1-60327-229-2_4