Epigenetic mechanisms in the regulation of the maize Suppressor-mutator transposon.

Ramesh Raina, M. Schläppi, N. Fedoroff

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Transcription and transposition of the maize Suppressor-mutator (Spm) transposon are epigenetically controlled. Methylation of specific element sequences prevents transcription and transposition in a heritable manner. Reactivation and demethylation occur in the presence of an active element, implying the existence of an element-encoded epigenetic activator. The methylation target sequences are the 0.2 kb promoter and an 0.35 kb GC-rich downstream sequence. Two Spm-encoded proteins, TnpA and TnpD, participate in transposition. In addition, TnpA has positive and negative regulatory activities. TnpA represses and activates the unmethylated and methylated Spm promoters, respectively, and it participates in the transient and heritable demethylation of the promoter and GC-rich region. There is evidence that TnpA-mediated repressor and epigenetic activator functions occur by different molecular mechanisms.

Original languageEnglish (US)
JournalNovartis Foundation Symposium
Volume214
StatePublished - 1998
Externally publishedYes

Fingerprint

GC Rich Sequence
Epigenomics
Methylation
Zea mays
Activator Appliances
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Epigenetic mechanisms in the regulation of the maize Suppressor-mutator transposon. / Raina, Ramesh; Schläppi, M.; Fedoroff, N.

In: Novartis Foundation Symposium, Vol. 214, 1998.

Research output: Contribution to journalArticle

@article{b19b4611355541d0bb749e7b43d6481a,
title = "Epigenetic mechanisms in the regulation of the maize Suppressor-mutator transposon.",
abstract = "Transcription and transposition of the maize Suppressor-mutator (Spm) transposon are epigenetically controlled. Methylation of specific element sequences prevents transcription and transposition in a heritable manner. Reactivation and demethylation occur in the presence of an active element, implying the existence of an element-encoded epigenetic activator. The methylation target sequences are the 0.2 kb promoter and an 0.35 kb GC-rich downstream sequence. Two Spm-encoded proteins, TnpA and TnpD, participate in transposition. In addition, TnpA has positive and negative regulatory activities. TnpA represses and activates the unmethylated and methylated Spm promoters, respectively, and it participates in the transient and heritable demethylation of the promoter and GC-rich region. There is evidence that TnpA-mediated repressor and epigenetic activator functions occur by different molecular mechanisms.",
author = "Ramesh Raina and M. Schl{\"a}ppi and N. Fedoroff",
year = "1998",
language = "English (US)",
volume = "214",
journal = "Novartis Foundation Symposium",
issn = "1528-2511",
publisher = "Novartis Foundation",

}

TY - JOUR

T1 - Epigenetic mechanisms in the regulation of the maize Suppressor-mutator transposon.

AU - Raina, Ramesh

AU - Schläppi, M.

AU - Fedoroff, N.

PY - 1998

Y1 - 1998

N2 - Transcription and transposition of the maize Suppressor-mutator (Spm) transposon are epigenetically controlled. Methylation of specific element sequences prevents transcription and transposition in a heritable manner. Reactivation and demethylation occur in the presence of an active element, implying the existence of an element-encoded epigenetic activator. The methylation target sequences are the 0.2 kb promoter and an 0.35 kb GC-rich downstream sequence. Two Spm-encoded proteins, TnpA and TnpD, participate in transposition. In addition, TnpA has positive and negative regulatory activities. TnpA represses and activates the unmethylated and methylated Spm promoters, respectively, and it participates in the transient and heritable demethylation of the promoter and GC-rich region. There is evidence that TnpA-mediated repressor and epigenetic activator functions occur by different molecular mechanisms.

AB - Transcription and transposition of the maize Suppressor-mutator (Spm) transposon are epigenetically controlled. Methylation of specific element sequences prevents transcription and transposition in a heritable manner. Reactivation and demethylation occur in the presence of an active element, implying the existence of an element-encoded epigenetic activator. The methylation target sequences are the 0.2 kb promoter and an 0.35 kb GC-rich downstream sequence. Two Spm-encoded proteins, TnpA and TnpD, participate in transposition. In addition, TnpA has positive and negative regulatory activities. TnpA represses and activates the unmethylated and methylated Spm promoters, respectively, and it participates in the transient and heritable demethylation of the promoter and GC-rich region. There is evidence that TnpA-mediated repressor and epigenetic activator functions occur by different molecular mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=0031632229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031632229&partnerID=8YFLogxK

M3 - Article

C2 - 9601015

AN - SCOPUS:0031632229

VL - 214

JO - Novartis Foundation Symposium

JF - Novartis Foundation Symposium

SN - 1528-2511

ER -