Enhanced detection of gold nanoparticles in agarose gel electrophoresis

Carina Hasenoehrl, Colleen M. Alexander, Nicholas N. Azzarelli, James C. Dabrowiak

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Gel electrophoresis is a powerful tool in gold nanoparticle (AuNP) research. While the technique is sensitive to the size, charge, and shape of particles, its optimal performance requires a relatively large amount of AuNP in the loading wells for visible detection of bands. We here describe a novel and more sensitive method for detecting AuNPs in agarose gels that involves staining the gel with the common organic fluorophore fluorescein, to produce AuNP band intensities that are linear with nanoparticle concentration and almost an order of magnitude larger than those obtained without staining the gel.

Original languageEnglish (US)
Pages (from-to)1251-1254
Number of pages4
JournalElectrophoresis
Volume33
Issue number8
DOIs
StatePublished - Apr 2012

Fingerprint

Agar Gel Electrophoresis
Electrophoresis
Gold
Nanoparticles
Sepharose
Gels
Staining and Labeling
Fluorophores
Fluorescein
Research

Keywords

  • Background staining
  • Gel electrophoresis
  • Gold nanoparticles

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Hasenoehrl, C., Alexander, C. M., Azzarelli, N. N., & Dabrowiak, J. C. (2012). Enhanced detection of gold nanoparticles in agarose gel electrophoresis. Electrophoresis, 33(8), 1251-1254. https://doi.org/10.1002/elps.201100556

Enhanced detection of gold nanoparticles in agarose gel electrophoresis. / Hasenoehrl, Carina; Alexander, Colleen M.; Azzarelli, Nicholas N.; Dabrowiak, James C.

In: Electrophoresis, Vol. 33, No. 8, 04.2012, p. 1251-1254.

Research output: Contribution to journalArticle

Hasenoehrl, C, Alexander, CM, Azzarelli, NN & Dabrowiak, JC 2012, 'Enhanced detection of gold nanoparticles in agarose gel electrophoresis', Electrophoresis, vol. 33, no. 8, pp. 1251-1254. https://doi.org/10.1002/elps.201100556
Hasenoehrl C, Alexander CM, Azzarelli NN, Dabrowiak JC. Enhanced detection of gold nanoparticles in agarose gel electrophoresis. Electrophoresis. 2012 Apr;33(8):1251-1254. https://doi.org/10.1002/elps.201100556
Hasenoehrl, Carina ; Alexander, Colleen M. ; Azzarelli, Nicholas N. ; Dabrowiak, James C. / Enhanced detection of gold nanoparticles in agarose gel electrophoresis. In: Electrophoresis. 2012 ; Vol. 33, No. 8. pp. 1251-1254.
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