Enhanced detection of gold nanoparticles in agarose gel electrophoresis

Carina Hasenoehrl, Colleen M. Alexander, Nicholas N. Azzarelli, James C. Dabrowiak

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Gel electrophoresis is a powerful tool in gold nanoparticle (AuNP) research. While the technique is sensitive to the size, charge, and shape of particles, its optimal performance requires a relatively large amount of AuNP in the loading wells for visible detection of bands. We here describe a novel and more sensitive method for detecting AuNPs in agarose gels that involves staining the gel with the common organic fluorophore fluorescein, to produce AuNP band intensities that are linear with nanoparticle concentration and almost an order of magnitude larger than those obtained without staining the gel.

Original languageEnglish (US)
Pages (from-to)1251-1254
Number of pages4
JournalElectrophoresis
Volume33
Issue number8
DOIs
StatePublished - Apr 1 2012

Keywords

  • Background staining
  • Gel electrophoresis
  • Gold nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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    Hasenoehrl, C., Alexander, C. M., Azzarelli, N. N., & Dabrowiak, J. C. (2012). Enhanced detection of gold nanoparticles in agarose gel electrophoresis. Electrophoresis, 33(8), 1251-1254. https://doi.org/10.1002/elps.201100556