Electrophoretic analysis of proteins from night-blind mutants of Phycomyces

J. A. Pollock, E. D. Lipson, D. T. Sullivan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Using two-dimensional gel electrophoresis, we have analyzed proteins from a plasma membrane-enriched fraction from Phycomyces sporangiophores. Specifically, we have compared gels for night-blind mutants and a wild-type strain to find proteins involved in the early steps of the sensory transduction chain for phototropism. In the gels for a mutant affected in the gene madA, a protein spot [51 kilodaltons (kdal) and pI 6.35] appears that is absent from the wild-type and the other mad mutants. Mutants affected in either of two madB alleles lack a protein spot (57 kdal and pI 6.6) that is present in the wild-type and all other mad strains; this spot probably represents the madB gene product. In some madC mutants, two spots (59 kdal, pI 6.5, with a covalently linked flavin; and 50 kdal, pI 6.4) are absent; however, in other madC strains, one or both of these spots are present. These four protein spots that are altered in madA, madB, and madC mutants may represent components of the photoreceptor complex responsible for phototropism in Phycomyces.

Original languageEnglish (US)
Pages (from-to)379-390
Number of pages12
JournalBiochemical Genetics
Volume23
Issue number5-6
DOIs
StatePublished - Jun 1 1985

Keywords

  • Phycomyces
  • blue-light photoreceptor
  • flavoprotein
  • night-blind mutants
  • phototropism

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

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