Effects of lead and mercury on the blood proteome of children

Robert E. Birdsall, Michael P. Kiley, Zaneer M. Segu, Christopher D. Palmer, Milan Madera, Brooks B Gump, James A. MacKenzie, Patrick J. Parsons, Yehia Mechref, Milos V. Novotny, Kestutis G. Bendinskas

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.

Original languageEnglish (US)
Pages (from-to)4443-4453
Number of pages11
JournalJournal of Proteome Research
Volume9
Issue number9
DOIs
StatePublished - Sep 3 2010
Externally publishedYes

Fingerprint

Proteome
Mercury
Blood
Apolipoproteins E
Electrophoresis
Proteins
Antibody Affinity
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Centers for Disease Control and Prevention (U.S.)
Medical problems
Heavy Metals
Tandem Mass Spectrometry
Proteomics
Mass spectrometry
Western Blotting
Genotype
Association reactions
Guidelines
Plasmas
Antibodies

Keywords

  • ApoE
  • apolipoprotein E
  • cardiovascular
  • children, blood
  • Hg
  • lead
  • mercury
  • Pb
  • proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Birdsall, R. E., Kiley, M. P., Segu, Z. M., Palmer, C. D., Madera, M., Gump, B. B., ... Bendinskas, K. G. (2010). Effects of lead and mercury on the blood proteome of children. Journal of Proteome Research, 9(9), 4443-4453. https://doi.org/10.1021/pr100204g

Effects of lead and mercury on the blood proteome of children. / Birdsall, Robert E.; Kiley, Michael P.; Segu, Zaneer M.; Palmer, Christopher D.; Madera, Milan; Gump, Brooks B; MacKenzie, James A.; Parsons, Patrick J.; Mechref, Yehia; Novotny, Milos V.; Bendinskas, Kestutis G.

In: Journal of Proteome Research, Vol. 9, No. 9, 03.09.2010, p. 4443-4453.

Research output: Contribution to journalArticle

Birdsall, RE, Kiley, MP, Segu, ZM, Palmer, CD, Madera, M, Gump, BB, MacKenzie, JA, Parsons, PJ, Mechref, Y, Novotny, MV & Bendinskas, KG 2010, 'Effects of lead and mercury on the blood proteome of children', Journal of Proteome Research, vol. 9, no. 9, pp. 4443-4453. https://doi.org/10.1021/pr100204g
Birdsall RE, Kiley MP, Segu ZM, Palmer CD, Madera M, Gump BB et al. Effects of lead and mercury on the blood proteome of children. Journal of Proteome Research. 2010 Sep 3;9(9):4443-4453. https://doi.org/10.1021/pr100204g
Birdsall, Robert E. ; Kiley, Michael P. ; Segu, Zaneer M. ; Palmer, Christopher D. ; Madera, Milan ; Gump, Brooks B ; MacKenzie, James A. ; Parsons, Patrick J. ; Mechref, Yehia ; Novotny, Milos V. ; Bendinskas, Kestutis G. / Effects of lead and mercury on the blood proteome of children. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 9. pp. 4443-4453.
@article{1521af8ef32243c98ee11e94803d10ca,
title = "Effects of lead and mercury on the blood proteome of children",
abstract = "Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.",
keywords = "ApoE, apolipoprotein E, cardiovascular, children, blood, Hg, lead, mercury, Pb, proteomics",
author = "Birdsall, {Robert E.} and Kiley, {Michael P.} and Segu, {Zaneer M.} and Palmer, {Christopher D.} and Milan Madera and Gump, {Brooks B} and MacKenzie, {James A.} and Parsons, {Patrick J.} and Yehia Mechref and Novotny, {Milos V.} and Bendinskas, {Kestutis G.}",
year = "2010",
month = "9",
day = "3",
doi = "10.1021/pr100204g",
language = "English (US)",
volume = "9",
pages = "4443--4453",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Effects of lead and mercury on the blood proteome of children

AU - Birdsall, Robert E.

AU - Kiley, Michael P.

AU - Segu, Zaneer M.

AU - Palmer, Christopher D.

AU - Madera, Milan

AU - Gump, Brooks B

AU - MacKenzie, James A.

AU - Parsons, Patrick J.

AU - Mechref, Yehia

AU - Novotny, Milos V.

AU - Bendinskas, Kestutis G.

PY - 2010/9/3

Y1 - 2010/9/3

N2 - Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.

AB - Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.

KW - ApoE

KW - apolipoprotein E

KW - cardiovascular

KW - children, blood

KW - Hg

KW - lead

KW - mercury

KW - Pb

KW - proteomics

UR - http://www.scopus.com/inward/record.url?scp=77956336164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956336164&partnerID=8YFLogxK

U2 - 10.1021/pr100204g

DO - 10.1021/pr100204g

M3 - Article

C2 - 20681587

AN - SCOPUS:77956336164

VL - 9

SP - 4443

EP - 4453

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 9

ER -