Effects of insert size on transposition efficiency of the Sleeping Beauty transposon in mouse cells

Attila Karsi, Boaz Moav, Perry Hackett, Zhanjiang Liu

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Transposon vectors are widely used in prokaryotic and lower eukaryotic systems. However, they were not available for use in vertebrate animals until the recent reconstitution of a synthetic fish transposon, Sleeping Beauty (SB). The reacquisition of transposability of the SB transposase fostered great enthusiasm for using transposon vectors as tools in vertebrate animals, particularly for gene transfer to facilitate accelerated integration of transgenes into chromosomes. Here, we report the effects of insert sizes on transposition efficiency of SB. A significant effect of insert size on efficiency of transposition by SB was found. The SB transposase enhanced the integration efficiency effectively for SB transposon up to approximately 5.6 kb, but lost its ability to enhance the integration efficiency when the transposon size was increased to 9.1 kb. This result indicates that the SB transposon system is highly applicable for transferring small genes, but may not be applicable for transferring very large genes.

Original languageEnglish (US)
Pages (from-to)241-245
Number of pages5
JournalMarine Biotechnology
Volume3
Issue number3
DOIs
StatePublished - Oct 20 2001
Externally publishedYes

Keywords

  • Gene transfer
  • Integration
  • Tcl-like
  • Transgenic fish

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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