Effects of ethanol on gastric epithelial cell phospholipid dynamics and cellular function

R. E. Bailey, R. A. Levine, J. Nandi, E. H. Schwartzel, D. H. Beach, P. N. Borer, G. C. Levy

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The lipid profile of isolated gastric superficial epithelial cells (SEC) was evaluated by proton nuclear magnetic resonance spectroscopy (1H-NMR). The most conspicuous resonance band in SEC spectra was due to the protons of +N(CH3)3 groups of phosphatidylcholine and, to a lesser degree, other phospholipid derivatives, on the basis of their chemical shift and addition of purified phospholipids. NMR of cell lysates and phospholipid extracts of SEC in deutero-chloroform provided further spectral resolution of these components. Phospholipase or ethanol treatments of SEC produced membrane disorganization reflected as increased peak intensity of the phospholipid signals. In addition, ethanol, in a dose-dependent manner, attenuated paranitrophenyl phosphatase activity, which correlated with inhibition of total and ouabain-sensitive 86Rubidium chloride uptake by SEC. This study suggests that NMR used in conjunction with other biochemical techniques can monitor SEC membrane structure-function relationships. NMR is a potentially powerful noninvasive probe to show changes in lipid membrane organization induced by low concentrations of ethanol (1%) and may indicate an early sign of 'cytotoxicity' in intact SEC.

Original languageEnglish (US)
Pages (from-to)G237-G243
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume252
Issue number2 (15/2)
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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    Bailey, R. E., Levine, R. A., Nandi, J., Schwartzel, E. H., Beach, D. H., Borer, P. N., & Levy, G. C. (1987). Effects of ethanol on gastric epithelial cell phospholipid dynamics and cellular function. American Journal of Physiology - Gastrointestinal and Liver Physiology, 252(2 (15/2)), G237-G243. https://doi.org/10.1152/ajpgi.1987.252.2.g237