TY - JOUR
T1 - Dynamic instability of native microtubules from squid axons is rare and independent of gliding and vesicle transport
AU - Seitz-Tutter, Dieter
AU - Langford, George M.
AU - Weiss, Dieter G.
N1 - Funding Information:
The excellent technical assistance of Monica A. Meyer, Wendy B. Green, and Petra Fegert is gratefully acknowledged. This work was supported by a DFG grant (We 790/l 1) to D. G. W. and NSF Grant DCB 851 7983 and a Nato Science Committee grant to G. M. L.
PY - 1988/10
Y1 - 1988/10
N2 - Dynamic instability characterizes the steady-state behavior of microtubules in vitro whereby polymer mass remains constant, while individual microtubules in the population may either grow or shrink. Video-enhanced contrast light microscopy was used to directly observe dynamic length changes in native, MAP-containing microtubules from squid axoplasm. We wanted to determine whether dynamic instability characterizes the steady-state behavior of axoplasmic microtubules in vitro. The lengths of a representative population of over 400 microtubules were analyzed. "Dynamic" microtubules were found to represent about 2 % of the population. This observation is different from that described for cultured cells or microtubules assembled from PC-purified tubulin where most microtubules were either growing or shrinking.
AB - Dynamic instability characterizes the steady-state behavior of microtubules in vitro whereby polymer mass remains constant, while individual microtubules in the population may either grow or shrink. Video-enhanced contrast light microscopy was used to directly observe dynamic length changes in native, MAP-containing microtubules from squid axoplasm. We wanted to determine whether dynamic instability characterizes the steady-state behavior of axoplasmic microtubules in vitro. The lengths of a representative population of over 400 microtubules were analyzed. "Dynamic" microtubules were found to represent about 2 % of the population. This observation is different from that described for cultured cells or microtubules assembled from PC-purified tubulin where most microtubules were either growing or shrinking.
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U2 - 10.1016/0014-4827(88)90418-1
DO - 10.1016/0014-4827(88)90418-1
M3 - Article
C2 - 2458953
AN - SCOPUS:0023727163
SN - 0014-4827
VL - 178
SP - 504
EP - 512
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -