Duplicated proteasome subunit genes in Drosophila melanogaster encoding testes-specific isoforms

Xiaoqing Yuan, Mary Miller, John M. Belote

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Using the previously cloned proteasome α-type subunit gene Pros28.1, we screened a Drosophila melanogaster genomic library using reduced stringency conditions to identify closely related genes. Two new genes, Pros28.1A (map position 92F) and Pros28.1B (map position 60D7), showing high sequence similarity to Pros28.1, were identified and characterized. Pros28.1A encodes a protein with 74% amino acid identity to PROS28.1 while the Pros28.1B gene product is 58% identical. The Pros28.1B gene has two introns, located in exactly analogous positions as the two introns in Pros28.1, while the Pros28.1A gene lacks introns. Northern blot analysis reveals that the two new genes are expressed only in males, during the pupal and adult stages. Tissue- specific patterns of expression were examined using transgenic flies carrying lacz-fusion reporter genes. This analysis revealed that both genes are expressed in germline cells during spermatogenesis, although their expression patterns differed. Pros28.1A expression is first detected at the primary spermatocyte stage and persists into the spermatid elongation phase of spermiogenesis, while Pros28.1B expression is prominent only during spermatid elongation. These genes represent the most striking example of cell-type- specific proteasome gene expression reported to date in any system and support the notion that their structural and functional heterogeneity among proteasomes in metazoans.

Original languageEnglish (US)
Pages (from-to)147-157
Number of pages11
JournalGenetics
Volume144
Issue number1
StatePublished - Sep 14 1996

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Duplicated proteasome subunit genes in Drosophila melanogaster encoding testes-specific isoforms'. Together they form a unique fingerprint.

  • Cite this