Distribution and diversity of Oligotrich and choreotrich ciliates assessed by morphology and DGGE in temperate coastal waters

Jean David Grattepanche, Luciana F. Santoferrara, Jessica Andrade, Angela M. Oliverio, George B. McManus, Laura A. Katz

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We assessed the diversity of coastal planktonic ciliates (Oligotrichia and Cho reo - trichia, Class: Spirotrichea) in Fishers Island Sound off the coast of Connecticut, USA using a combination of denaturant gradient gel electrophoresis (DGGE) and morphological analyses. To test the hypothesis that ciliate communities followed water masses, we sampled 3 times at a surface drifter (i.e. floating buoy) and twice at intervening times from points 1 km away. By repeating both PCRs and gel electrophoresis under varying conditions, we confirmed that DGGE is an appropriate tool to capture the ciliate community composition, based on the high repeatability of samples. The 2 methods used for assessing ciliate community structure showed similar levels of diversity for tintinnids. However, there was a mismatch in diversity estimates for the aloricate ciliates, especially for the oligotrichs where the estimated diversity was greater by DGGE than by microscopy. This may be attributable to biases in the DGGE (e.g. primer or gene copy number issues) and/or misidentification by morphology. However, the pattern of community structure assessed by cluster analyses is similar for both microscopy and DGGE, suggesting that the mismatch between these methods does not introduce bias in biogeographical analysis. Analyses of both DGGE haplotypes and microscopically observed morphospecies revealed that the ciliate communities were not static within the water mass. Community structure and abundance patterns varied with salinity and currents. These changes could be due to mixing with adjacent estuarine and coastal assemblages, ciliate behavior, and/or other factors that cause assemblages to be dynamic over short (i.e. ultradian) periods.

Original languageEnglish (US)
Pages (from-to)211-221
Number of pages11
JournalAquatic Microbial Ecology
Issue number3
StatePublished - 2014
Externally publishedYes


  • Choreotrichia
  • Ciliate assemblage
  • DGGE
  • Morphology
  • Oligotrichia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science


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