Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo

Bin Deng, Colin Wright, Eric Lewis-Clark, G. Shaheen, Roman Geier, J. Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

13 Scopus citations

Abstract

Human transdermal in vivo spectroscopic applications for tissue analysis involving near infrared (NIR) light often must contend with broadband NIR fluorescence that, depending on what kind of spectroscopy is being employed, can degrade signal to noise ratios and dynamic range. Such NIR fluorescence, i.e. "autofluorescence" is well known to originate in blood tissues and various other endogenous materials associated with the static tissues. Results of recent experiments on human volar side fingertips in vivo are beginning to provide a relative ordering of the contributions from various sources. Preliminary results involving the variation in the bleaching effect across different individuals suggest that for 830 nm excitation well over half of the total fluorescence comes from the static tissues and remainder originates with the blood tissues, i.e. the plasma and the hematocrit. Of the NIR fluorescence associated with the static tissue, over half originates with products of well-known post-enzymatic glycation reactions, i.e. Maillard chemistry, in the skin involving glucose and other carbohydrates and skin proteins like collagen and cytosol proteins.

Original languageEnglish (US)
Title of host publicationBiomedical Vibrational Spectroscopy IV
Subtitle of host publicationAdvances in Research and Industry
DOIs
StatePublished - May 7 2010
EventBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7560
ISSN (Print)1605-7422

Other

OtherBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Keywords

  • Autofluorescence
  • In vivo
  • Near infrared
  • Noninvasive
  • Photobleaching
  • Raman

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Deng, B., Wright, C., Lewis-Clark, E., Shaheen, G., Geier, R., & Chaiken, J. (2010). Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo. In Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry [75600P] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7560). https://doi.org/10.1117/12.846900