Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo

Bin Deng, Colin Wright, Eric Lewis-Clark, G. Shaheen, Roman Geier, Joseph Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

Human transdermal in vivo spectroscopic applications for tissue analysis involving near infrared (NIR) light often must contend with broadband NIR fluorescence that, depending on what kind of spectroscopy is being employed, can degrade signal to noise ratios and dynamic range. Such NIR fluorescence, i.e. "autofluorescence" is well known to originate in blood tissues and various other endogenous materials associated with the static tissues. Results of recent experiments on human volar side fingertips in vivo are beginning to provide a relative ordering of the contributions from various sources. Preliminary results involving the variation in the bleaching effect across different individuals suggest that for 830 nm excitation well over half of the total fluorescence comes from the static tissues and remainder originates with the blood tissues, i.e. the plasma and the hematocrit. Of the NIR fluorescence associated with the static tissue, over half originates with products of well-known post-enzymatic glycation reactions, i.e. Maillard chemistry, in the skin involving glucose and other carbohydrates and skin proteins like collagen and cytosol proteins.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7560
DOIs
StatePublished - 2010
EventBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherBiomedical Vibrational Spectroscopy IV: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

Photobleaching
Observation
Tissue
Infrared radiation
Fluorescence
fluorescence
blood
Skin
Blood
hematocrit
proteins
Proteins
Maillard Reaction
carbohydrates
Signal-To-Noise Ratio
bleaching
collagens
Carbohydrates
Bleaching
Hematocrit

Keywords

  • Autofluorescence
  • In vivo
  • Near infrared
  • Noninvasive
  • Photobleaching
  • Raman

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Deng, B., Wright, C., Lewis-Clark, E., Shaheen, G., Geier, R., & Chaiken, J. (2010). Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7560). [75600P] https://doi.org/10.1117/12.846900

Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo. / Deng, Bin; Wright, Colin; Lewis-Clark, Eric; Shaheen, G.; Geier, Roman; Chaiken, Joseph.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7560 2010. 75600P.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Deng, B, Wright, C, Lewis-Clark, E, Shaheen, G, Geier, R & Chaiken, J 2010, Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7560, 75600P, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.846900
Deng B, Wright C, Lewis-Clark E, Shaheen G, Geier R, Chaiken J. Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7560. 2010. 75600P https://doi.org/10.1117/12.846900
Deng, Bin ; Wright, Colin ; Lewis-Clark, Eric ; Shaheen, G. ; Geier, Roman ; Chaiken, Joseph. / Direct noninvasive observation of near infrared photobleaching of autofluorescence in human volar side fingertips in vivo. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7560 2010.
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