Direct attachment of oligonucleotides to quantum dot interfaces

Hyunjoo Han, Joshua Zylstra, Mathew M Maye

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A straightforward functionalization strategy for the direct attachment of single-stranded oligonucleotides (ssDNA) to quantum dot (qdot) interfaces is described. The approach takes advantage of a histidine-mediated phase transfer protocol that results in qdots with high colloidal stability in aqueous buffers. The weakly bound histidine encapsulation facilitates monolayer exchanged with both thiolated ssDNA and polyhistidine-tagged proteins. The successful biomodification at the qdot interface was probed by FRET analysis. The modest FRET efficiencies measured suggest the DNA to be in an extended conformation that is the result of high surface coverage that the direct attachment provides.

Original languageEnglish (US)
Pages (from-to)4975-4981
Number of pages7
JournalChemistry of Materials
Volume23
Issue number22
DOIs
StatePublished - Nov 22 2011

Fingerprint

Oligonucleotides
Histidine
Semiconductor quantum dots
Encapsulation
Conformations
Monolayers
Buffers
DNA
Proteins
polyhistidine

Keywords

  • DNA
  • FRET
  • histidine
  • Qdot FRET
  • quantum dot
  • Self-assembly

ASJC Scopus subject areas

  • Materials Chemistry
  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Direct attachment of oligonucleotides to quantum dot interfaces. / Han, Hyunjoo; Zylstra, Joshua; Maye, Mathew M.

In: Chemistry of Materials, Vol. 23, No. 22, 22.11.2011, p. 4975-4981.

Research output: Contribution to journalArticle

Han, Hyunjoo ; Zylstra, Joshua ; Maye, Mathew M. / Direct attachment of oligonucleotides to quantum dot interfaces. In: Chemistry of Materials. 2011 ; Vol. 23, No. 22. pp. 4975-4981.
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