Development of polymorphic EST markers suitable for genetic linkage mapping of catfish

Zhanjiang Liu, Attila Karsi, Rex A. Dunham

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Expressed sequence tag (EST) markers are important for gene mapping and for marker-assisted selection (MAS). To develop EST markers for use in catfish gene mapping, 100 randomly picked complementary DNAs from the channel catfish (Ictalurus punctatus) pituitary library were sequenced. The EST sequences were used to design primers to amplify channel catfish and blue catfish (I. furcatus) genomic DNAs. Polymerase chain reaction products of the ESTs were analyzed to determine length polymorphism between the channel catfish and blue catfish. Eleven polymorphic EST markers were identified. Five of the 11 EST markers were from known genes and the other six were from unidentified ESTs. Seven ESTs were found to be associated with microsatellite sequences. Analysis of channel catfish gene sequences indicated highly biased codon usage, with 16 codons being preferably used. These codons were more preferably used in highly expressed ribosomal protein genes and in highly expressed pituitary hormone genes. G/C-rich codons are less used in channel catfish than those in other vertebrates suggesting AT-richness of the channel catfish genome.

Original languageEnglish (US)
Pages (from-to)437-447
Number of pages11
JournalMarine Biotechnology
Volume1
Issue number5
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Catfish
  • Expressed sequence tags
  • Gene mapping
  • Marker
  • Polymorphism

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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