Development of expression vectors for transgenic fish

Zhanjiang Liu, Boaz Moav, Anthony J. Faras, Kevin S. Guise, Anne R. Kapuscinski, Perry B. Hackett

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Genetic alteration of fish is important for aquatic biotechnology as well as for investigating molecular interactions that occur during vertebrate development. The numerous, large, transparent, and externally fertilized eggs of many fish species make them ideally suitable for genetic manipulation, especially for production of transgenic animals. Genetic engineering of fish requires suitable expression vectors. Accordingly, we developed two fish expression vectors, FV-1 and FV-2, which contain the proximal promoter and enhancer regulatory elements of the carp ß-actin gene and the polyadenylation signal from the salmon growth hormone gene. The two fish expression vectors were tested in mi-croinjected fish eggs and in tissue cultured fish and mammalian cells. These two “all-fish” expression vectors should be useful for genetic engineering of fish and have been used with growth-enhancing genes in transgenic fish.

Original languageEnglish (US)
Pages (from-to)1268-1272
Number of pages5
JournalBio/Technology
Volume8
Issue number12
DOIs
StatePublished - Dec 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology

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    Liu, Z., Moav, B., Faras, A. J., Guise, K. S., Kapuscinski, A. R., & Hackett, P. B. (1990). Development of expression vectors for transgenic fish. Bio/Technology, 8(12), 1268-1272. https://doi.org/10.1038/nbt1290-1268