TY - JOUR
T1 - Development of expression vectors for transgenic fish
AU - Liu, Zhanjiang
AU - Moav, Boaz
AU - Faras, Anthony J.
AU - Guise, Kevin S.
AU - Kapuscinski, Anne R.
AU - Hackett, Perry B.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1990/12
Y1 - 1990/12
N2 - Genetic alteration of fish is important for aquatic biotechnology as well as for investigating molecular interactions that occur during vertebrate development. The numerous, large, transparent, and externally fertilized eggs of many fish species make them ideally suitable for genetic manipulation, especially for production of transgenic animals. Genetic engineering of fish requires suitable expression vectors. Accordingly, we developed two fish expression vectors, FV-1 and FV-2, which contain the proximal promoter and enhancer regulatory elements of the carp ß-actin gene and the polyadenylation signal from the salmon growth hormone gene. The two fish expression vectors were tested in mi-croinjected fish eggs and in tissue cultured fish and mammalian cells. These two “all-fish” expression vectors should be useful for genetic engineering of fish and have been used with growth-enhancing genes in transgenic fish.
AB - Genetic alteration of fish is important for aquatic biotechnology as well as for investigating molecular interactions that occur during vertebrate development. The numerous, large, transparent, and externally fertilized eggs of many fish species make them ideally suitable for genetic manipulation, especially for production of transgenic animals. Genetic engineering of fish requires suitable expression vectors. Accordingly, we developed two fish expression vectors, FV-1 and FV-2, which contain the proximal promoter and enhancer regulatory elements of the carp ß-actin gene and the polyadenylation signal from the salmon growth hormone gene. The two fish expression vectors were tested in mi-croinjected fish eggs and in tissue cultured fish and mammalian cells. These two “all-fish” expression vectors should be useful for genetic engineering of fish and have been used with growth-enhancing genes in transgenic fish.
UR - http://www.scopus.com/inward/record.url?scp=14744271779&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14744271779&partnerID=8YFLogxK
U2 - 10.1038/nbt1290-1268
DO - 10.1038/nbt1290-1268
M3 - Article
C2 - 1366961
AN - SCOPUS:14744271779
VL - 8
SP - 1268
EP - 1272
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 12
ER -