TY - JOUR
T1 - Development of amplified fragment length polymorphism (AFLP) markers suitable for genetic linkage mapping of catfish
AU - Liu, Zhanjiang
AU - Li, Ping
AU - Kucuktas, Huseyin
AU - Nichols, Amy
AU - Tan, Guo
AU - Zheng, Xinmin
AU - Argue, Brad J.
AU - Dunham, Rex A.
N1 - Funding Information:
This project was supported by the Department of Fisheries and Allied Aquacultures, the College of Agriculture and the Alabama Agricultural Experiment Station, and the Office of the Vice President for Research of Auburn University (grant 233078 to Z.J.L.), by Auburn University Competitive Research Grant 213966 to Z.J.L., by a U.S. Department of Agriculture National Research Initiative Competitive Grants Program grant (98-35205-6738) to Z.J.L., and in part by a grant from Gold Kist Aquaculture Research Center to Z.J.L. and R.A.D. This paper is journal number 8-985908 of the Alabama Agricultural Experiment Station.
PY - 1999/3
Y1 - 1999/3
N2 - Genomic research requires many molecular markers for construction of the genetic linkage map and for marker-assisted selection (MAS). Amplified fragment length polymorphism (AFLP) markers are inherited with Mendelian expectations in catfish and, thus, are suitable for use in gene mapping and MAS. To identify large numbers of AFLP markers, 64 primer combinations were tested to generate AFLP patterns between channel catfish Ictalurus punctatus and blue catfish I. furcatus. The 64 primer combinations amplified 7,871 bands, of which 3,081 were polymorphic between the two mating parents of channel catfish and blue catfish used to produce the reference families for mapping analysis. The primer combinations that produced fewer bands showed higher rates of polymorphism. Of the 64 primers, 53 primer combinations produced clean and reproducible AFLP profiles. Nine primer combinations produced fair AFLP profiles. The remaining two primers produced poor AFLP profiles with intensive background bands. The 53 excellent primer combinations produced 2,572 AFLP markers. These markers are suitable for use in gene and quantitative trait loci mapping of catfish by using the interspecific hybrid system. Analysis of genetic variations among strains of channel catfish indicated that AFLP should also be a valuable tool for genetic resource and population analyses in fish.
AB - Genomic research requires many molecular markers for construction of the genetic linkage map and for marker-assisted selection (MAS). Amplified fragment length polymorphism (AFLP) markers are inherited with Mendelian expectations in catfish and, thus, are suitable for use in gene mapping and MAS. To identify large numbers of AFLP markers, 64 primer combinations were tested to generate AFLP patterns between channel catfish Ictalurus punctatus and blue catfish I. furcatus. The 64 primer combinations amplified 7,871 bands, of which 3,081 were polymorphic between the two mating parents of channel catfish and blue catfish used to produce the reference families for mapping analysis. The primer combinations that produced fewer bands showed higher rates of polymorphism. Of the 64 primers, 53 primer combinations produced clean and reproducible AFLP profiles. Nine primer combinations produced fair AFLP profiles. The remaining two primers produced poor AFLP profiles with intensive background bands. The 53 excellent primer combinations produced 2,572 AFLP markers. These markers are suitable for use in gene and quantitative trait loci mapping of catfish by using the interspecific hybrid system. Analysis of genetic variations among strains of channel catfish indicated that AFLP should also be a valuable tool for genetic resource and population analyses in fish.
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U2 - 10.1577/1548-8659(1999)128<0317:DOAFLP>2.0.CO;2
DO - 10.1577/1548-8659(1999)128<0317:DOAFLP>2.0.CO;2
M3 - Article
AN - SCOPUS:0033057098
SN - 0002-8487
VL - 128
SP - 317
EP - 327
JO - Transactions of the American Fisheries Society
JF - Transactions of the American Fisheries Society
IS - 2
ER -