Designing quantum rods for optimized energy transfer with firefly luciferase enzymes

Rabeka Alam, Danielle M. Fontaine, Bruce R. Branchini, Mathew M Maye

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.

Original languageEnglish (US)
Pages (from-to)3251-3256
Number of pages6
JournalNano Letters
Volume12
Issue number6
DOIs
StatePublished - Jun 13 2012

Fingerprint

fireflies
Bioluminescence
Firefly Luciferases
Energy transfer
enzymes
rods
Enzymes
energy transfer
Microstructure
Surface chemistry
Aspect ratio
bioluminescence
microstructure
aspect ratio
chemistry

Keywords

  • biotic/abiotic
  • BRET
  • energy transfer
  • quantum rods

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Mechanical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Designing quantum rods for optimized energy transfer with firefly luciferase enzymes. / Alam, Rabeka; Fontaine, Danielle M.; Branchini, Bruce R.; Maye, Mathew M.

In: Nano Letters, Vol. 12, No. 6, 13.06.2012, p. 3251-3256.

Research output: Contribution to journalArticle

Alam, Rabeka ; Fontaine, Danielle M. ; Branchini, Bruce R. ; Maye, Mathew M. / Designing quantum rods for optimized energy transfer with firefly luciferase enzymes. In: Nano Letters. 2012 ; Vol. 12, No. 6. pp. 3251-3256.
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