Designing quantum rods for optimized energy transfer with firefly luciferase enzymes

Rabeka Alam; Danielle M. Fontaine; Bruce R. Branchini; Mathew M. Maye

doi:10.1021/nl301291g

Designing quantum rods for optimized energy transfer with firefly luciferase enzymes

Rabeka Alam, Danielle M. Fontaine, Bruce R. Branchini, Mathew M. Maye

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.

Original language	English (US)
Pages (from-to)	3251-3256
Number of pages	6
Journal	Nano Letters
Volume	12
Issue number	6
DOIs	https://doi.org/10.1021/nl301291g
State	Published - Jun 13 2012

Keywords

BRET
biotic/abiotic
energy transfer
quantum rods

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl301291g

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title = "Designing quantum rods for optimized energy transfer with firefly luciferase enzymes",

abstract = "The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.",

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AU - Alam, Rabeka

AU - Fontaine, Danielle M.

AU - Branchini, Bruce R.

AU - Maye, Mathew M.

PY - 2012/6/13

Y1 - 2012/6/13

N2 - The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.

AB - The bioluminescence resonance energy transfer (BRET) between firefly luciferase from Photinus pyralis (Ppy) with core/shell semiconductive quantum rods (QRs) has been studied as a function of QR aspect ratio and internal microstructure. The QRs were found to be ideal energy acceptors, and Ppy-to-core distances were optimized using rod-in-rod microstructures that were achieved by the synthetic control of rod morphology, surface chemistry, and Ppy:QR loading. The BRET ratios of >44 measured are the highest efficiencies to date.

KW - BRET

KW - biotic/abiotic

KW - energy transfer

KW - quantum rods

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JO - Nano Letters

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Designing quantum rods for optimized energy transfer with firefly luciferase enzymes

Abstract

Keywords

ASJC Scopus subject areas

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