Designing mechanical and electrical properties of microtubules to modulate gliding trajectories

N. Isozaki, H. Shintaku, H. Kotera, T. L. Hawkins, J. L. Ross, R. Yokokawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Toward a multiplex molecular sorter, we propose a method to design electrical charge and mechanical stiffness of microtubules (MTs) for controlling trajectories of kinesin-propelled MTs regarded as molecular transporters in a microfluidic device. Seven MTs having different properties-persistence length (Lp) and surface charge density-were polymerized, and investigated the relationship between their properties and trajectories under a given electric field. We determined the optimal MT combination and device structure, leading to sort two MT groups with ∼85% precision in a PDMS device. Additive effects of mechanical and electrical properties achieved a high-precision MT sorting.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages120-121
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - Jan 1 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • Elecrical charge
  • Kinesin
  • Microtubule
  • Molecular sorter
  • PDMS
  • Persistence length

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Isozaki, N., Shintaku, H., Kotera, H., Hawkins, T. L., Ross, J. L., & Yokokawa, R. (2016). Designing mechanical and electrical properties of microtubules to modulate gliding trajectories. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 120-121). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.