Design of catalytically amplified sensors for small molecules

Research output: Contribution to journalReview articlepeer-review

17 Scopus citations


Catalytically amplified sensors link an allosteric analyte binding site with a reactive site to catalytically convert substrate into colored or fluorescent product that can be easily measured. Such an arrangement greatly improves a sensor's detection limit as illustrated by successful application of ELISA-based approaches. The ability to engineer synthetic catalytic sites into non-enzymatic proteins expands the repertoire of analytes as well as readout reactions. Here we review recent examples of small molecule sensors based on allosterically controlled enzymes and organometallic catalysts. The focus of this paper is on biocompatible, switchable enzymes regulated by small molecules to track analytes both in vivo and in the environment.

Original languageEnglish (US)
Pages (from-to)402-418
Number of pages17
Issue number2
StatePublished - 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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