@article{3753dd3e44f148bbbe6b7450ab7f84c9,
title = "Design and Synthesis of an Alkynyl Luciferin Analogue for Bioluminescence Imaging",
abstract = "Herein, the synthesis and characterization of an alkyne-modified luciferin is reported. This bioluminescent probe was accessed using C-H activation methodology and was found to be stable in solution and capable of light production with firefly luciferase. The luciferin analogue was also cell permeant and emitted more redshifted light than d-luciferin, the native luciferase substrate. Based on these features, the alkynyl luciferin will be useful for a variety of imaging applications.",
keywords = "C-H activation, Sonogashira coupling, bioluminescence imaging, luciferase, luciferin",
author = "Steinhardt, {Rachel C.} and O'Neill, {Jessica M.} and Rathbun, {Colin M.} and McCutcheon, {David C.} and Paley, {Miranda A.} and Prescher, {Jennifer A.}",
note = "Funding Information: This work was supported by the National Institutes of Health (NIH, R01M107630 to J.A.P.). Some experiments were performed in the Laboratory for Fluorescence Dynamics (LFD) at UC Irvine. The LFD is supported jointly by the National Institute of General Medical Sciences of the NIH (8P41M103540) and UC Irvine. We also thank members of the Jarvo, Chamberlin, and Overman laboratories for providing reagents and experimental advice. We thank Krysten Jones for kindly preparing samples for cellular assays. Finally, we thank members of the Prescher laboratory for assistance with manuscript preparations. Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2016",
month = mar,
day = "7",
doi = "10.1002/chem.201503944",
language = "English (US)",
volume = "22",
pages = "3671--3675",
journal = "Chemistry - A European Journal",
issn = "0947-6539",
publisher = "Wiley-VCH Verlag",
number = "11",
}