Decellularized extracellular matrix microparticles as a vehicle for cellular delivery in a model of anastomosis healing

David M. Hoganson, Gwen E. Owens, Amanda M. Meppelink, Erik K. Bassett, Chris M. Bowley, Cameron J. Hinkel, Eric B. Finkelstein, Scott M. Goldman, Joseph P. Vacanti

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Extracellular matrix (ECM) materials from animal and human sources have become important materials for soft tissue repair. Microparticles of ECM materials have increased surface area and exposed binding sites compared to sheet materials. Decellularized porcine peritoneum was mechanically dissociated into 200 μm microparticles, seeded with fibroblasts and cultured in a low gravity rotating bioreactor. The cells avidly attached and maintained excellent viability on the microparticles. When the seeded microparticles were placed in a collagen gel, the cells quickly migrated off the microparticles and through the gel. Cells from seeded microparticles migrated to and across an in vitro anastomosis model, increasing the tensile strength of the model. Cell seeded microparticles of ECM material have potential for paracrine and cellular delivery therapies when delivered in a gel carrier.

Original languageEnglish (US)
Pages (from-to)1728-1735
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume104
Issue number7
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Keywords

  • 3-D cell culture
  • cell adhesion
  • cell migration
  • extracellular matrix
  • microparticles

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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