Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit

Wen Zhi Gai; Shu Man Sun; Nobuhito Sone; Samuel H.P. Chan

doi:10.1016/0006-291X(90)90347-P

Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit

Wen Zhi Gai, Shu Man Sun, Nobuhito Sone, Samuel H.P. Chan

Department of Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. "Western" blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.

Original language	English (US)
Pages (from-to)	414-421
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)90347-P
State	Published - Jun 15 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit",

abstract = "Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. {"}Western{"} blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.",

author = "Gai, {Wen Zhi} and Sun, {Shu Man} and Nobuhito Sone and Chan, {Samuel H.P.}",

note = "Funding Information: This study is supported by NIH grant GM-32246 and a grant-in-aid from American Heart Association, New York State Affiliate. We thank Dr. J. Foster and C. Rich for determining amino acid compositions.",

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language = "English (US)",

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T1 - Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit

AU - Gai, Wen Zhi

AU - Sun, Shu Man

AU - Sone, Nobuhito

AU - Chan, Samuel H.P.

N1 - Funding Information: This study is supported by NIH grant GM-32246 and a grant-in-aid from American Heart Association, New York State Affiliate. We thank Dr. J. Foster and C. Rich for determining amino acid compositions.

PY - 1990/6/15

Y1 - 1990/6/15

N2 - Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. "Western" blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.

AB - Monoclonal antibodies prepared against subunits II and IV of beef heart cytochrome oxidase were found to cross-react with thermophilic bacterial PS3 oxidase. Each individual antibody affects the enzymatic activity. "Western" blot analyses showed that subunit II antibodies of beef heart recognized subunit II of PS3 and subunit IV antibody likewise recognized a fourth protein subunit on slab gels. This fourth subunit previously thought to be a contaminant or a degradation product has a molecular weight of about 10,500 on SDS-gels, and appears to exist in stoichiometric amount. We have extracted this subunit from slab gels and compared its amino acid composition with that of subunit III.

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Cytochrome oxidase from thermophilic bacterium PS3 contains a fourth protein subunit

Abstract

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