Current noise of a protein-selective biological nanopore

Jiaxin Sun, Avinash Kumar Thakur, Liviu Movileanu

Research output: Contribution to journalArticlepeer-review

Abstract

1/f current noise is ubiquitous in protein pores, porins, and channels. We have previously shown that a protein-selective biological nanopore with an external protein receptor can function as a 1/f noise generator when a high-affinity protein ligand is reversibly captured by the receptor. Here, we demonstrate that the binding affinity and concentration of the ligand are key determinants for the nature of current noise. For example, 1/f was absent when a protein ligand was reversibly captured at a much lower concentration than its equilibrium dissociation constant against the receptor. Furthermore, we also analyzed the composite current noise that resulted from mixtures of low-affinity and high-affinity ligands against the same receptor. This study highlights the significance of protein recognition events in the current noise fluctuations across biological membranes.

Original languageEnglish (US)
JournalProteomics
DOIs
StateAccepted/In press - 2021

Keywords

  • FhuA
  • ion channel
  • membrane protein engineering
  • protein detection, protein dynamics
  • single-molecule electrophysiology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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