Crystallographic data collection using a 0.22% bandwidth multilayer

Ulrich Englich, Alexander Kazimirov, Qun Shen, Don H. Bilderback, Sol M. Gruner, Quan Hao

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


To bridge the gap between traditional multilayer and crystal optics a high-resolution multilayer monochromator with a bandwidth of 0.22% has been designed and installed on a bending-magnet beamline (F3) at the Cornell High Energy Synchrotron Source (CHESS) to provide an unfocused monochromatic X-ray beam for protein crystallography experiments. Crystallographic data of excellent quality from a medium-sized protein, Concanavalin A, were collected and processed using standard Crystallographic programs. The data were successfully used for a structure solution and refinement. The flux from the multilayer monochromator is enhanced, relative to that from a flat Si(111) monochromator, by a factor of 5; consequently, data collection is faster and/or smaller samples may be used. At the same time, the bandwidth is narrow enough to avoid streaked spots. This experiment suggests that multilayer optics may play a valuable role in satisfying the demands of the structural biology community for rapid X-ray data collection, particularly at under-utilized bending-magnet beamlines.

Original languageEnglish (US)
Pages (from-to)345-348
Number of pages4
JournalJournal of Synchrotron Radiation
Issue number3
StatePublished - May 2005


  • Macromolecular crystallography
  • Monochromators
  • Multilayer optics
  • Protein crystallography
  • X-ray optics

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation


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