Cryogel bioreactor for therapeutic protein production

Era Jain, Ashok Kumar

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Disposable bioreactors are becoming popular for small-scale therapeutic development and initial clinical trials due to their low cost and high efficiency. Our group has developed disposable cryogel bioreactor matrices for therapeutic protein production. A distinguishing feature of these cryogel bioreactor matrices is their low cost, a high surface-to-volume ratio and effective nutrient transport. Polyacrylamide coupled with gelatin or as a semi-interpenetrating network with chitosan (polyacrylamide-chitosan) has been used to formulate these cryogel bioreactor matrices. Both matrices are synthesized by free radical polymerization at -12°C in various formats such as monolith, beads and discs. Both adherent and nonadherent cells can be immobilized over a cryogel surface in high density for therapeutics production. The packed-bed, closed, continuous bioreactor configuration has been used to produce therapeutically important proteins like urokinase or monoclonal antibodies (mAb). The bioreactor can also be run as an integrated setup where protein purification can be done online with production using an affinity cryogel. The efficiency of these bioreactors has been found to be four times that of T-flask. Furthermore, the cryogels have also been used successfully to culture primary hepatocytes and hepatocarcinoma cell lines to be used in a bioreactor matrix in bioartificial liver devices (BAL).

Original languageEnglish (US)
Title of host publicationSupermacroporous Cryogels
Subtitle of host publicationBiomedical and Biotechnological Applications
PublisherCRC Press
Pages419-443
Number of pages25
ISBN (Electronic)9781482228823
ISBN (Print)9781482228816
StatePublished - Apr 6 2016

Keywords

  • Bioprocessing
  • Bioreactor
  • Monoclonal antibody
  • Polyacrylamide-chitosan cryogel
  • Therapeutic protein production

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Materials Science(all)

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  • Cite this

    Jain, E., & Kumar, A. (2016). Cryogel bioreactor for therapeutic protein production. In Supermacroporous Cryogels: Biomedical and Biotechnological Applications (pp. 419-443). CRC Press.