Creation and testing of a new, local microtubule-disruption tool based on the microtubule-severing enzyme, katanin p60

Siddheshwari Advani, Thomas J. Maresca, Jennifer L. Ross

Research output: Contribution to journalArticle

Abstract

Current methods to disrupt the microtubule cytoskeleton do not easily provide rapid, local control with standard cell manipulation reagents. Here, we develop a new microtubule-disruption tool based on katanin p60 severing activity and demonstrate proof-of-principle by targeting it to kinetochores in Drosophila melanogaster S2 cells. Specifically, we show that human katanin p60 can remove microtubule polymer mass in S2 cells and an increase in misaligned chromosomes when globally overexpressed. When katanin p60 was targeted to the kinetochores via Mis12, we were able to recapitulate the misalignment only when using a phosphorylation-resistant mutant katanin p60. Our results demonstrate that targeting an active version of katanin p60 to the kinetochore can reduce the fidelity of achieving full chromosome alignment in metaphase and could serve as a microtubule disruption tool for the future.

Original languageEnglish (US)
Pages (from-to)531-544
Number of pages14
JournalCytoskeleton
Volume75
Issue number12
DOIs
StatePublished - Dec 2018

Keywords

  • cell cycle
  • fidgetin
  • laser ablation
  • mitosis
  • nocodazole
  • spastin
  • vinblastin

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

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