Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups

Carlos A. Castañeda, Jia Liu, Tanuja R. Kashyap, Rajesh K. Singh, David Fushman, T. Ashton Cropp

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

E2 enzymes catalyze the ATP-dependent polymerization of polyubiquitin chains which function as molecular signals in the regulation of numerous cellular processes. Here we present a method that uses genetically encoded unnatural amino acids to halt and re-start ubiquitin polymerization providing access to natural-linkage, precision-length ubiquitin chains that can be used for biochemical, structural, and dynamics studies.

Original languageEnglish (US)
Pages (from-to)2026-2028
Number of pages3
JournalChemical Communications
Volume47
Issue number7
DOIs
StatePublished - Feb 1 2011

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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