Controllable g5p-protein-directed aggregation of ssDNA-gold nanoparticles

Soo Kwan Lee, Mathew M. Maye, Yian Biao Zhang, Oleg Gang, Daniel Van Der Lelie

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


We assembled single-stranded DNA (ssDNA) conjugated nanoparticles using the phage M13 gene 5 protein (g5p) as the molecular glue to bind two antiparallel noncomplementary ssDNA strands. The entire process was controlled tightly by the concentration of the g5p protein and the presence of double-stranded DNA. The g5p-ssDNA aggregate was disintegrated by hybridization with complementary ssDNA (C-ssDNA) that triggers the dissociation of the complex. Polyhistidine-tagged g5p was bound to nickel nitrilotriacetic acid (Ni 2+-NTA) conjugated nanoparticles and subsequently used to coassemble the ssDNA-conjugated nanoparticles into multiparticle-type aggregates. Our approach offers great promise for designing biologically functional, controllable protein/nanoparticle composites.

Original languageEnglish (US)
Pages (from-to)657-660
Number of pages4
Issue number2
StatePublished - Jan 20 2009
Externally publishedYes

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


Dive into the research topics of 'Controllable g5p-protein-directed aggregation of ssDNA-gold nanoparticles'. Together they form a unique fingerprint.

Cite this