Controllable g5p-protein-directed aggregation of ssDNA-gold nanoparticles

Soo Kwan Lee, Mathew M Maye, Yian Biao Zhang, Oleg Gang, Daniel Van Der Lelie

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We assembled single-stranded DNA (ssDNA) conjugated nanoparticles using the phage M13 gene 5 protein (g5p) as the molecular glue to bind two antiparallel noncomplementary ssDNA strands. The entire process was controlled tightly by the concentration of the g5p protein and the presence of double-stranded DNA. The g5p-ssDNA aggregate was disintegrated by hybridization with complementary ssDNA (C-ssDNA) that triggers the dissociation of the complex. Polyhistidine-tagged g5p was bound to nickel nitrilotriacetic acid (Ni 2+-NTA) conjugated nanoparticles and subsequently used to coassemble the ssDNA-conjugated nanoparticles into multiparticle-type aggregates. Our approach offers great promise for designing biologically functional, controllable protein/nanoparticle composites.

Original languageEnglish (US)
Pages (from-to)657-660
Number of pages4
JournalLangmuir
Volume25
Issue number2
DOIs
StatePublished - Jan 20 2009
Externally publishedYes

Fingerprint

Single-Stranded DNA
Gold
genes
DNA
Agglomeration
deoxyribonucleic acid
Genes
gold
Nanoparticles
proteins
Proteins
nanoparticles
Bacteriophages
glues
Glues
strands
actuators
Nickel
nickel
dissociation

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Controllable g5p-protein-directed aggregation of ssDNA-gold nanoparticles. / Lee, Soo Kwan; Maye, Mathew M; Zhang, Yian Biao; Gang, Oleg; Van Der Lelie, Daniel.

In: Langmuir, Vol. 25, No. 2, 20.01.2009, p. 657-660.

Research output: Contribution to journalArticle

Lee, SK, Maye, MM, Zhang, YB, Gang, O & Van Der Lelie, D 2009, 'Controllable g5p-protein-directed aggregation of ssDNA-gold nanoparticles', Langmuir, vol. 25, no. 2, pp. 657-660. https://doi.org/10.1021/la803596q
Lee, Soo Kwan ; Maye, Mathew M ; Zhang, Yian Biao ; Gang, Oleg ; Van Der Lelie, Daniel. / Controllable g5p-protein-directed aggregation of ssDNA-gold nanoparticles. In: Langmuir. 2009 ; Vol. 25, No. 2. pp. 657-660.
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