Contributions of primary coordination ligands and importance of outer sphere interactions in UFsc, a de novo designed protein with high affinity for metal ions

Alona Kulesha, Jennifer H. Yoon, Cara Chester, Areetha D'Souza, Christos Costeas, Olga V. Makhlynets

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Metalloproteins constitute nearly half of all proteins and catalyze some of the most complex chemical reactions. Recently, we reported a design of 4G-UFsc (Uno Ferro single chain), a single chain four-helical bundle with extraordinarily high (30 pM) affinity for zinc. We evaluated the contribution of different side chains to binding of Co(II), Ni(II), Zn(II) and Mn(II) using systematic mutagenesis of the amino acids that constitute the primary metal coordination and outer spheres. The binding affinity of proteins for metals was then measured using isothermal titration calorimetry. Our results show that both primary metal coordination environment and side chains in the outer sphere of UFsc are highly sensitive to even slight changes and can be adapted to binding different 3d metals, including hard-to-tightly bind metal ions such as Mn(II). The studies on the origins of tight metal binding will guide future metalloprotein design efforts.

Original languageEnglish (US)
Article number111224
JournalJournal of Inorganic Biochemistry
Volume212
DOIs
StatePublished - Nov 2020

Keywords

  • De novo protein design
  • Isothermal titration calorimetry
  • Mag-Fura 2
  • Zinc binding

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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